Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath
Abstract We performed a sequential morphological and molecular biological study of the development of the vertebral bodies in A tlantic salmon ( S almo salar L .). Mineralization starts in separate bony elements which fuse to form complete segmental rings within the notochord sheath. The nucleation...
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crwiley:10.1111/joa.12067 2024-09-15T17:56:32+00:00 Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath Wang, Shou Kryvi, Harald Grotmol, Sindre Wargelius, Anna Krossøy, Christel Epple, Mattias Neues, Frank Furmanek, Tomasz Totland, Geir K. Research Council of Norway 2013 http://dx.doi.org/10.1111/joa.12067 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjoa.12067 https://onlinelibrary.wiley.com/doi/pdf/10.1111/joa.12067 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Anatomy volume 223, issue 2, page 159-170 ISSN 0021-8782 1469-7580 journal-article 2013 crwiley https://doi.org/10.1111/joa.12067 2024-08-09T04:31:45Z Abstract We performed a sequential morphological and molecular biological study of the development of the vertebral bodies in A tlantic salmon ( S almo salar L .). Mineralization starts in separate bony elements which fuse to form complete segmental rings within the notochord sheath. The nucleation and growth of hydroxyapatite crystals in both the lamellar type II collagen matrix of the notochord sheath and the lamellar type I collagen matrix derived from the sclerotome, were highly similar. In both matrices the hydroxyapatite crystals nucleate and accrete on the surface of the collagen fibrils rather than inside the fibrils, a process that may be controlled by a template imposed by the collagen fibrils. Apatite crystal growth starts with the formation of small plate‐like structures, about 5 nm thick, that gradually grow and aggregate to form extensive multi‐branched crystal arborizations, resembling dendritic growth. The hydroxyapatite crystals are always oriented parallel to the long axis of the collagen fibrils, and the lamellar collagen matrices provide oriented support for crystal growth. We demonstrate here for the first time by means of synchroton radiation based on X‐ray diffraction that the chordacentra contain hydroxyapatite. We employed quantitative real‐time PCR to study the expression of key signalling molecule transcripts expressed in the cellular core of the notochord. The results indicate that the notochord not only produces and maintains the notochord sheath but also expresses factors known to regulate skeletogenesis: sonic hedgehog ( shh ), indian hedgehog homolog b ( ihhb ), parathyroid hormone 1 receptor ( pth1r ) and transforming growth factor beta 1 ( tgfb1 ). In conclusion, our study provides evidence for the process of vertebral body development in teleost fishes, which is initially orchestrated by the notochord. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Anatomy 223 2 159 170 |
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Wiley Online Library |
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English |
description |
Abstract We performed a sequential morphological and molecular biological study of the development of the vertebral bodies in A tlantic salmon ( S almo salar L .). Mineralization starts in separate bony elements which fuse to form complete segmental rings within the notochord sheath. The nucleation and growth of hydroxyapatite crystals in both the lamellar type II collagen matrix of the notochord sheath and the lamellar type I collagen matrix derived from the sclerotome, were highly similar. In both matrices the hydroxyapatite crystals nucleate and accrete on the surface of the collagen fibrils rather than inside the fibrils, a process that may be controlled by a template imposed by the collagen fibrils. Apatite crystal growth starts with the formation of small plate‐like structures, about 5 nm thick, that gradually grow and aggregate to form extensive multi‐branched crystal arborizations, resembling dendritic growth. The hydroxyapatite crystals are always oriented parallel to the long axis of the collagen fibrils, and the lamellar collagen matrices provide oriented support for crystal growth. We demonstrate here for the first time by means of synchroton radiation based on X‐ray diffraction that the chordacentra contain hydroxyapatite. We employed quantitative real‐time PCR to study the expression of key signalling molecule transcripts expressed in the cellular core of the notochord. The results indicate that the notochord not only produces and maintains the notochord sheath but also expresses factors known to regulate skeletogenesis: sonic hedgehog ( shh ), indian hedgehog homolog b ( ihhb ), parathyroid hormone 1 receptor ( pth1r ) and transforming growth factor beta 1 ( tgfb1 ). In conclusion, our study provides evidence for the process of vertebral body development in teleost fishes, which is initially orchestrated by the notochord. |
author2 |
Research Council of Norway |
format |
Article in Journal/Newspaper |
author |
Wang, Shou Kryvi, Harald Grotmol, Sindre Wargelius, Anna Krossøy, Christel Epple, Mattias Neues, Frank Furmanek, Tomasz Totland, Geir K. |
spellingShingle |
Wang, Shou Kryvi, Harald Grotmol, Sindre Wargelius, Anna Krossøy, Christel Epple, Mattias Neues, Frank Furmanek, Tomasz Totland, Geir K. Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
author_facet |
Wang, Shou Kryvi, Harald Grotmol, Sindre Wargelius, Anna Krossøy, Christel Epple, Mattias Neues, Frank Furmanek, Tomasz Totland, Geir K. |
author_sort |
Wang, Shou |
title |
Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
title_short |
Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
title_full |
Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
title_fullStr |
Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
title_full_unstemmed |
Mineralization of the vertebral bodies in Atlantic salmon ( Salmo salar L.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
title_sort |
mineralization of the vertebral bodies in atlantic salmon ( salmo salar l.) is initiated segmentally in the form of hydroxyapatite crystal accretions in the notochord sheath |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/joa.12067 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjoa.12067 https://onlinelibrary.wiley.com/doi/pdf/10.1111/joa.12067 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Anatomy volume 223, issue 2, page 159-170 ISSN 0021-8782 1469-7580 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/joa.12067 |
container_title |
Journal of Anatomy |
container_volume |
223 |
container_issue |
2 |
container_start_page |
159 |
op_container_end_page |
170 |
_version_ |
1810432731875639296 |