Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane
Abstract Synthesis of isoamyl acetate by mechanical and ultrasonic solvent‐free agitations using Candida antarctica lipase B (CALB) as biocatalyst immobilized in polyurethane was studied. Temperature (24.8–75.2 °C), ultrasonic power (35–123 W; only in ultrasonic system) and molar ratio of substrates...
Published in: | Journal of Food Process Engineering |
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crwiley:10.1111/jfpe.12812 2024-09-15T17:46:14+00:00 Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane Nyari, Nádia Paulazzi, Alessandro Zamadei, Raquel Steffens, Clarice Zabot, Giovani Leone Tres, Marcus Vinícius Zeni, Jamile Venquiaruto, Luciana Dallago, Rogério Marcos Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Universidade Federal de Santa Maria 2018 http://dx.doi.org/10.1111/jfpe.12812 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfpe.12812 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfpe.12812 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Process Engineering volume 41, issue 6 ISSN 0145-8876 1745-4530 journal-article 2018 crwiley https://doi.org/10.1111/jfpe.12812 2024-08-09T04:28:29Z Abstract Synthesis of isoamyl acetate by mechanical and ultrasonic solvent‐free agitations using Candida antarctica lipase B (CALB) as biocatalyst immobilized in polyurethane was studied. Temperature (24.8–75.2 °C), ultrasonic power (35–123 W; only in ultrasonic system) and molar ratio of substrates (1:3.64–1:10.36) were the parameters evaluated. The responses taken into account were the acetic acid conversion in the esterification reaction (21–95%) and the residual conversion of substrates after successive reuse cycles of CALB. Ultrasonic system was advantageous because the substrate conversions into isoamyl acetate were reached six times earlier than in mechanical system. An increase in the temperature and ultrasonic power and a reduction in acetic acid : isoamyl alcohol ratio favored an increase in conversion yields. CALB used in ultrasonic reactions maintained more than 60% of its residual activity after 27 reuse cycles, which was more than thrice (eight reuse cycles) when using CALB in mechanical reactions. Practical applications The practical uses of isoamyl acetate are mainly as a food additive and as a solvent in the aircraft industry. In food‐related areas, this ester is used to confer characteristics such as banana (or pear) smell and flavor in foods and beverages. It has a pleasant odor and low toxicity, which encourages its widespread use in different receipts, including in the formulation of beers. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Food Process Engineering 41 6 e12812 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Synthesis of isoamyl acetate by mechanical and ultrasonic solvent‐free agitations using Candida antarctica lipase B (CALB) as biocatalyst immobilized in polyurethane was studied. Temperature (24.8–75.2 °C), ultrasonic power (35–123 W; only in ultrasonic system) and molar ratio of substrates (1:3.64–1:10.36) were the parameters evaluated. The responses taken into account were the acetic acid conversion in the esterification reaction (21–95%) and the residual conversion of substrates after successive reuse cycles of CALB. Ultrasonic system was advantageous because the substrate conversions into isoamyl acetate were reached six times earlier than in mechanical system. An increase in the temperature and ultrasonic power and a reduction in acetic acid : isoamyl alcohol ratio favored an increase in conversion yields. CALB used in ultrasonic reactions maintained more than 60% of its residual activity after 27 reuse cycles, which was more than thrice (eight reuse cycles) when using CALB in mechanical reactions. Practical applications The practical uses of isoamyl acetate are mainly as a food additive and as a solvent in the aircraft industry. In food‐related areas, this ester is used to confer characteristics such as banana (or pear) smell and flavor in foods and beverages. It has a pleasant odor and low toxicity, which encourages its widespread use in different receipts, including in the formulation of beers. |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Universidade Federal de Santa Maria |
format |
Article in Journal/Newspaper |
author |
Nyari, Nádia Paulazzi, Alessandro Zamadei, Raquel Steffens, Clarice Zabot, Giovani Leone Tres, Marcus Vinícius Zeni, Jamile Venquiaruto, Luciana Dallago, Rogério Marcos |
spellingShingle |
Nyari, Nádia Paulazzi, Alessandro Zamadei, Raquel Steffens, Clarice Zabot, Giovani Leone Tres, Marcus Vinícius Zeni, Jamile Venquiaruto, Luciana Dallago, Rogério Marcos Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
author_facet |
Nyari, Nádia Paulazzi, Alessandro Zamadei, Raquel Steffens, Clarice Zabot, Giovani Leone Tres, Marcus Vinícius Zeni, Jamile Venquiaruto, Luciana Dallago, Rogério Marcos |
author_sort |
Nyari, Nádia |
title |
Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
title_short |
Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
title_full |
Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
title_fullStr |
Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
title_full_unstemmed |
Synthesis of isoamyl acetate by ultrasonic system using Candida antarctica lipase B immobilized in polyurethane |
title_sort |
synthesis of isoamyl acetate by ultrasonic system using candida antarctica lipase b immobilized in polyurethane |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jfpe.12812 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfpe.12812 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfpe.12812 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Food Process Engineering volume 41, issue 6 ISSN 0145-8876 1745-4530 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfpe.12812 |
container_title |
Journal of Food Process Engineering |
container_volume |
41 |
container_issue |
6 |
container_start_page |
e12812 |
_version_ |
1810494237116989440 |