MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming
Abstract A Multi‐Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa , which causes ‘winter ulcer’ predominantly in sea‐reared Atlantic salmon ( Salmo salar L.). The assay involves multiplex PCR amplification of si...
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Online Access: | http://dx.doi.org/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13766 |
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crwiley:10.1111/jfd.13766 2024-04-14T08:09:17+00:00 MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming Sørgaard, Martin Sveinsson, Karoline Patel, Sonal Nilsen, Hanne K. Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan J. Gulla, Snorre Norges Forskningsråd Skretting Aquaculture Research Centre 2023 http://dx.doi.org/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13766 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Fish Diseases volume 46, issue 5, page 535-543 ISSN 0140-7775 1365-2761 Veterinary (miscellaneous) Aquatic Science journal-article 2023 crwiley https://doi.org/10.1111/jfd.13766 2024-03-19T10:54:59Z Abstract A Multi‐Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa , which causes ‘winter ulcer’ predominantly in sea‐reared Atlantic salmon ( Salmo salar L.). The assay involves multiplex PCR amplification of six Variable Number of Tandem Repeat (VNTR) loci, followed by capillary electrophoresis and data interpretation. A collection of 747 spatiotemporally diverse M. viscosa isolates from nine fish species was analysed, the majority from farmed Norwegian salmon. MLVA distributed 76% of the isolates across three major clonal complexes (CC1, CC2 and CC3), with the remaining forming minor clusters and singletons. While 90% of the salmon isolates belong to either CC1, CC2 or CC3, only 20% of the isolates recovered from other fish species do so, indicating a considerable degree of host specificity. We further highlight a series of ‘clonal shifts’ amongst Norwegian salmon isolates over the 35‐year sampling period, with CC1 showing exclusive predominance prior to the emergence of CC2, which was later supplanted by CC3, before the recent re‐emergence of CC1. Apparently, these shifts have rapidly swept the entire Norwegian coastline and conceivably, as suggested by typing of a small number of non‐Norwegian isolates, the Northeast Atlantic region as a whole. Article in Journal/Newspaper Atlantic salmon Northeast Atlantic Salmo salar Wiley Online Library Journal of Fish Diseases 46 5 535 543 |
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English |
topic |
Veterinary (miscellaneous) Aquatic Science |
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Veterinary (miscellaneous) Aquatic Science Sørgaard, Martin Sveinsson, Karoline Patel, Sonal Nilsen, Hanne K. Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan J. Gulla, Snorre MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
topic_facet |
Veterinary (miscellaneous) Aquatic Science |
description |
Abstract A Multi‐Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa , which causes ‘winter ulcer’ predominantly in sea‐reared Atlantic salmon ( Salmo salar L.). The assay involves multiplex PCR amplification of six Variable Number of Tandem Repeat (VNTR) loci, followed by capillary electrophoresis and data interpretation. A collection of 747 spatiotemporally diverse M. viscosa isolates from nine fish species was analysed, the majority from farmed Norwegian salmon. MLVA distributed 76% of the isolates across three major clonal complexes (CC1, CC2 and CC3), with the remaining forming minor clusters and singletons. While 90% of the salmon isolates belong to either CC1, CC2 or CC3, only 20% of the isolates recovered from other fish species do so, indicating a considerable degree of host specificity. We further highlight a series of ‘clonal shifts’ amongst Norwegian salmon isolates over the 35‐year sampling period, with CC1 showing exclusive predominance prior to the emergence of CC2, which was later supplanted by CC3, before the recent re‐emergence of CC1. Apparently, these shifts have rapidly swept the entire Norwegian coastline and conceivably, as suggested by typing of a small number of non‐Norwegian isolates, the Northeast Atlantic region as a whole. |
author2 |
Norges Forskningsråd Skretting Aquaculture Research Centre |
format |
Article in Journal/Newspaper |
author |
Sørgaard, Martin Sveinsson, Karoline Patel, Sonal Nilsen, Hanne K. Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan J. Gulla, Snorre |
author_facet |
Sørgaard, Martin Sveinsson, Karoline Patel, Sonal Nilsen, Hanne K. Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan J. Gulla, Snorre |
author_sort |
Sørgaard, Martin |
title |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
title_short |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
title_full |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
title_fullStr |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
title_full_unstemmed |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in Norwegian salmon farming |
title_sort |
mlva genotyping of moritella viscosa reveals serial emergence of novel, host‐specific clonal complexes in norwegian salmon farming |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13766 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13766 |
genre |
Atlantic salmon Northeast Atlantic Salmo salar |
genre_facet |
Atlantic salmon Northeast Atlantic Salmo salar |
op_source |
Journal of Fish Diseases volume 46, issue 5, page 535-543 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/jfd.13766 |
container_title |
Journal of Fish Diseases |
container_volume |
46 |
container_issue |
5 |
container_start_page |
535 |
op_container_end_page |
543 |
_version_ |
1796306769525342208 |