<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture
Abstract Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To invest...
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Online Access: | http://dx.doi.org/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13656 |
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crwiley:10.1111/jfd.13656 2024-04-14T08:09:16+00:00 <scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture Riborg, Andreas Gulla, Snorre Strand, David Wiik‐Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan J. Fiskeri - og havbruksnæringens forskningsfond Norges Forskningsråd U.S. Department of Agriculture 2022 http://dx.doi.org/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13656 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ Journal of Fish Diseases volume 45, issue 8, page 1211-1224 ISSN 0140-7775 1365-2761 Veterinary (miscellaneous) Aquatic Science journal-article 2022 crwiley https://doi.org/10.1111/jfd.13656 2024-03-19T10:57:00Z Abstract Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To investigate the presence and spread of virulent and putatively avirulent strains in Norwegian salmon farms, PCR assays specific for Y. ruckeri (species level) and Y. ruckeri clonal complex 1 were developed. Following extensive screening of water and biofilm, the widespread prevalence of putatively avirulent Y. ruckeri strains was confirmed in freshwater salmon hatcheries, while Y. ruckeri clonal complex 1 was found in fewer farms. The formalin‐killed bacterin yersiniosis vaccine was detected in environmental samples by both PCR assays for several weeks post‐vaccination. It is thus important to interpret results from recently vaccinated fish with great care. Moreover, field studies and laboratory trials confirmed that stressful management procedures may result in increased shedding of Y. ruckeri by sub‐clinically infected fish. Analysis of sea water sampled throughout thermal delousing procedures proved effective for detection of Y. ruckeri in sub‐clinically infected populations. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Norway Journal of Fish Diseases 45 8 1211 1224 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Veterinary (miscellaneous) Aquatic Science |
spellingShingle |
Veterinary (miscellaneous) Aquatic Science Riborg, Andreas Gulla, Snorre Strand, David Wiik‐Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan J. <scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
topic_facet |
Veterinary (miscellaneous) Aquatic Science |
description |
Abstract Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To investigate the presence and spread of virulent and putatively avirulent strains in Norwegian salmon farms, PCR assays specific for Y. ruckeri (species level) and Y. ruckeri clonal complex 1 were developed. Following extensive screening of water and biofilm, the widespread prevalence of putatively avirulent Y. ruckeri strains was confirmed in freshwater salmon hatcheries, while Y. ruckeri clonal complex 1 was found in fewer farms. The formalin‐killed bacterin yersiniosis vaccine was detected in environmental samples by both PCR assays for several weeks post‐vaccination. It is thus important to interpret results from recently vaccinated fish with great care. Moreover, field studies and laboratory trials confirmed that stressful management procedures may result in increased shedding of Y. ruckeri by sub‐clinically infected fish. Analysis of sea water sampled throughout thermal delousing procedures proved effective for detection of Y. ruckeri in sub‐clinically infected populations. |
author2 |
Fiskeri - og havbruksnæringens forskningsfond Norges Forskningsråd U.S. Department of Agriculture |
format |
Article in Journal/Newspaper |
author |
Riborg, Andreas Gulla, Snorre Strand, David Wiik‐Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan J. |
author_facet |
Riborg, Andreas Gulla, Snorre Strand, David Wiik‐Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan J. |
author_sort |
Riborg, Andreas |
title |
<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_short |
<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_full |
<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_fullStr |
<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_full_unstemmed |
<scp>qPCR</scp> screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_sort |
<scp>qpcr</scp> screening for yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in norwegian aquaculture |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13656 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13656 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases volume 45, issue 8, page 1211-1224 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/jfd.13656 |
container_title |
Journal of Fish Diseases |
container_volume |
45 |
container_issue |
8 |
container_start_page |
1211 |
op_container_end_page |
1224 |
_version_ |
1796306756671897600 |