Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis)
Abstract Salmonid diseases caused by infections of Flavobacterium psychrophilum , the causative agent of bacterial coldwater disease, remain difficult to manage as novel, pathogenic strains continue to emerge in aquaculture settings globally. To date, much of the research regarding treatment options...
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crwiley:10.1111/jfd.13349 2024-06-23T07:51:13+00:00 Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) Bruce, Timothy J. Ma, Jie Jones, Evan M. Vuglar, Brent M. Oliver, Luke P. Knupp, Christopher Loch, Thomas P. Cain, Kenneth D. 2021 http://dx.doi.org/10.1111/jfd.13349 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13349 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13349 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 44, issue 5, page 645-653 ISSN 0140-7775 1365-2761 journal-article 2021 crwiley https://doi.org/10.1111/jfd.13349 2024-06-13T04:24:12Z Abstract Salmonid diseases caused by infections of Flavobacterium psychrophilum , the causative agent of bacterial coldwater disease, remain difficult to manage as novel, pathogenic strains continue to emerge in aquaculture settings globally. To date, much of the research regarding treatment options and vaccine development has focused on rainbow trout ( Oncorhynchus mykiss ), but other inland‐reared salmonids are also impacted by this Gram‐negative bacterium. As such, Atlantic salmon ( Salmo salar ) and brook trout ( Salvelinus fontinalis ) were injection‐challenged with a variety of previously reported F. psychrophilum strains isolated from disease diagnostic cases in salmonids, as well as a standard and well‐studied F. psychrophilum strain (CSF 259–93) known to be virulent in rainbow trout. In three separate virulence assessments (Trials A, B and C), strains US063 (isolated from lake trout; Salvelinus namaycush ) and US149 (isolated from Atlantic salmon) caused a significantly higher cumulative per cent mortality (CPM) relative to other strains in Atlantic salmon ( p <.001 for all trials), with US149 causing significantly greater mortality than US063 in Trials A (CPM 97% vs. 65%, p =.008) and B (CPM 96% ± 2.3% vs. 81.33% ± 4.8%, p =.014). Trial C used a lower dose (1.86 × 10 8 CFU/mL) for US149, resulting in a lower mortality (78.67% ± 9.33%) relative to Trials A and B. CSF259‐93 did not cause significant mortality in any trials. In brook trout, the strain 03–179 (originally isolated from steelhead trout; Oncorhynchus mykiss ) was significantly more virulent than any other (CPM 100% ± 0%, p <.001), followed by US063 (73% ± 3.8%) and US149 (40% ± 6.1%,) respectively. Again, CSF259‐93 did not cause significant mortality relative to a mock challenge treatment. Results provide information about the applicability of strain selection in F. psychrophilum virulence testing in Atlantic salmon and brook trout, demonstrating the high virulence of US063 and US149 for these salmonid species. This information is ... Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 44 5 645 653 |
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English |
description |
Abstract Salmonid diseases caused by infections of Flavobacterium psychrophilum , the causative agent of bacterial coldwater disease, remain difficult to manage as novel, pathogenic strains continue to emerge in aquaculture settings globally. To date, much of the research regarding treatment options and vaccine development has focused on rainbow trout ( Oncorhynchus mykiss ), but other inland‐reared salmonids are also impacted by this Gram‐negative bacterium. As such, Atlantic salmon ( Salmo salar ) and brook trout ( Salvelinus fontinalis ) were injection‐challenged with a variety of previously reported F. psychrophilum strains isolated from disease diagnostic cases in salmonids, as well as a standard and well‐studied F. psychrophilum strain (CSF 259–93) known to be virulent in rainbow trout. In three separate virulence assessments (Trials A, B and C), strains US063 (isolated from lake trout; Salvelinus namaycush ) and US149 (isolated from Atlantic salmon) caused a significantly higher cumulative per cent mortality (CPM) relative to other strains in Atlantic salmon ( p <.001 for all trials), with US149 causing significantly greater mortality than US063 in Trials A (CPM 97% vs. 65%, p =.008) and B (CPM 96% ± 2.3% vs. 81.33% ± 4.8%, p =.014). Trial C used a lower dose (1.86 × 10 8 CFU/mL) for US149, resulting in a lower mortality (78.67% ± 9.33%) relative to Trials A and B. CSF259‐93 did not cause significant mortality in any trials. In brook trout, the strain 03–179 (originally isolated from steelhead trout; Oncorhynchus mykiss ) was significantly more virulent than any other (CPM 100% ± 0%, p <.001), followed by US063 (73% ± 3.8%) and US149 (40% ± 6.1%,) respectively. Again, CSF259‐93 did not cause significant mortality relative to a mock challenge treatment. Results provide information about the applicability of strain selection in F. psychrophilum virulence testing in Atlantic salmon and brook trout, demonstrating the high virulence of US063 and US149 for these salmonid species. This information is ... |
format |
Article in Journal/Newspaper |
author |
Bruce, Timothy J. Ma, Jie Jones, Evan M. Vuglar, Brent M. Oliver, Luke P. Knupp, Christopher Loch, Thomas P. Cain, Kenneth D. |
spellingShingle |
Bruce, Timothy J. Ma, Jie Jones, Evan M. Vuglar, Brent M. Oliver, Luke P. Knupp, Christopher Loch, Thomas P. Cain, Kenneth D. Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
author_facet |
Bruce, Timothy J. Ma, Jie Jones, Evan M. Vuglar, Brent M. Oliver, Luke P. Knupp, Christopher Loch, Thomas P. Cain, Kenneth D. |
author_sort |
Bruce, Timothy J. |
title |
Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
title_short |
Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
title_full |
Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
title_fullStr |
Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
title_full_unstemmed |
Assessment of Flavobacterium psychrophilum‐associated mortality in Atlantic salmon ( Salmo salar) and brook trout ( Salvelinus fontinalis) |
title_sort |
assessment of flavobacterium psychrophilum‐associated mortality in atlantic salmon ( salmo salar) and brook trout ( salvelinus fontinalis) |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/jfd.13349 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13349 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13349 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 44, issue 5, page 645-653 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.13349 |
container_title |
Journal of Fish Diseases |
container_volume |
44 |
container_issue |
5 |
container_start_page |
645 |
op_container_end_page |
653 |
_version_ |
1802642237628088320 |