Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss)
Abstract Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and tro...
Published in: | Journal of Fish Diseases |
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crwiley:10.1111/jfd.13182 2024-09-30T14:32:27+00:00 Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) Purcell, Maureen K. Powers, Rachel L. Taksdal, Torunn McKenney, Doug Conway, Carla M. Elliott, Diane G. Polinski, Mark Garver, Kyle Winton, James U.S. Geological Survey 2020 http://dx.doi.org/10.1111/jfd.13182 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.13182 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13182 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13182 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ Journal of Fish Diseases volume 43, issue 7, page 719-728 ISSN 0140-7775 1365-2761 journal-article 2020 crwiley https://doi.org/10.1111/jfd.13182 2024-09-17T04:45:22Z Abstract Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon and rainbow trout injected with tissue homogenates from Atlantic salmon testing positive for PRV‐1 or with control material. Fish were sampled at intervals to assess viral RNA transcript levels, haematocrit, erythrocytic inclusions and histopathology. While PRV‐1 replicated in all species, there was negligible mortality in any group. We observed a few erythrocytic inclusion bodies in fish from the PRV‐1‐infected groups. At a few time points, haematocrits were significantly lower in the PRV‐1‐infected groups relative to controls, but in no case was anaemia noted. The most common histopathological finding was mild, focal myocarditis in both the non‐infected controls and PRV‐1‐infected fish. All cardiac lesions were judged mild, and none were consistent with those of HSMI. Together, these results suggest all three species are susceptible to PRV‐1 infection, but in no case did infection cause notable disease in these experiments. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Pacific Journal of Fish Diseases 43 7 719 728 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Piscine orthoreovirus genotype 1 (PRV‐1) is the causative agent of heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon ( Salmo salar L.). The virus has also been found in Pacific salmonids in western North America, raising concerns about the risk to native salmon and trout. Here, we report the results of laboratory challenges using juvenile Chinook salmon, coho salmon and rainbow trout injected with tissue homogenates from Atlantic salmon testing positive for PRV‐1 or with control material. Fish were sampled at intervals to assess viral RNA transcript levels, haematocrit, erythrocytic inclusions and histopathology. While PRV‐1 replicated in all species, there was negligible mortality in any group. We observed a few erythrocytic inclusion bodies in fish from the PRV‐1‐infected groups. At a few time points, haematocrits were significantly lower in the PRV‐1‐infected groups relative to controls, but in no case was anaemia noted. The most common histopathological finding was mild, focal myocarditis in both the non‐infected controls and PRV‐1‐infected fish. All cardiac lesions were judged mild, and none were consistent with those of HSMI. Together, these results suggest all three species are susceptible to PRV‐1 infection, but in no case did infection cause notable disease in these experiments. |
author2 |
U.S. Geological Survey |
format |
Article in Journal/Newspaper |
author |
Purcell, Maureen K. Powers, Rachel L. Taksdal, Torunn McKenney, Doug Conway, Carla M. Elliott, Diane G. Polinski, Mark Garver, Kyle Winton, James |
spellingShingle |
Purcell, Maureen K. Powers, Rachel L. Taksdal, Torunn McKenney, Doug Conway, Carla M. Elliott, Diane G. Polinski, Mark Garver, Kyle Winton, James Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
author_facet |
Purcell, Maureen K. Powers, Rachel L. Taksdal, Torunn McKenney, Doug Conway, Carla M. Elliott, Diane G. Polinski, Mark Garver, Kyle Winton, James |
author_sort |
Purcell, Maureen K. |
title |
Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
title_short |
Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
title_full |
Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
title_fullStr |
Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
title_full_unstemmed |
Consequences of Piscine orthoreovirus genotype 1 (PRV‐1) infections in Chinook salmon ( Oncorhynchus tshawytscha), coho salmon ( O. kisutch) and rainbow trout ( O. mykiss) |
title_sort |
consequences of piscine orthoreovirus genotype 1 (prv‐1) infections in chinook salmon ( oncorhynchus tshawytscha), coho salmon ( o. kisutch) and rainbow trout ( o. mykiss) |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/jfd.13182 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.13182 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13182 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13182 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 43, issue 7, page 719-728 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/jfd.13182 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
7 |
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719 |
op_container_end_page |
728 |
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1811636613212536832 |