Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream
Abstract Enteromyxoses are relevant diseases for turbot and gilthead sea bream aquaculture. The myxozoan parasites invade the intestinal mucosa, causing a cachectic syndrome associated with intestinal barrier alteration; nonetheless, their pathological impact is different. Turbot infected by Enterom...
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Online Access: | http://dx.doi.org/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13130 |
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crwiley:10.1111/jfd.13130 2024-09-15T18:39:59+00:00 Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream Ronza, Paolo Estensoro, Itziar Bermúdez, Roberto Losada, Ana Paula Pérez‐Cordón, Gregorio Pardo, Belén G. Sitjà‐Bobadilla, Ariadna Quiroga, Mª Isabel Generalitat Valenciana Horizon 2020 Framework Programme Ministerio de Ciencia e Innovación 2020 http://dx.doi.org/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13130 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 43, issue 3, page 337-346 ISSN 0140-7775 1365-2761 journal-article 2020 crwiley https://doi.org/10.1111/jfd.13130 2024-07-23T04:14:30Z Abstract Enteromyxoses are relevant diseases for turbot and gilthead sea bream aquaculture. The myxozoan parasites invade the intestinal mucosa, causing a cachectic syndrome associated with intestinal barrier alteration; nonetheless, their pathological impact is different. Turbot infected by Enteromyxum scophthalmi develop more severe intestinal lesions, reaching mortality rates of 100%, whereas in E. leei ‐infected gilthead sea bream, the disease progresses slowly, and mortality rates are lower. The mechanisms underlying the different pathogenesis are still unclear. We studied the distribution and expression changes of E‐cadherin, a highly conserved protein of the adherens junctions, in the intestine of both species by immunohistochemistry and quantitative PCR, using the same immunohistochemical protocol and common primers. The regular immunostaining pattern observed in control fish turned into markedly irregular in parasitized turbot, showing an intense immunoreaction at the host–parasite interface. Nevertheless, E‐cadherin gene expression was not significantly modulated in this species. On the contrary, no evident changes in the protein distribution were noticed in gilthead sea bream, whereas a significant gene downregulation occurred in advanced infection. The results contribute to the understanding of the different host–parasite interactions in enteromyxoses. Host and parasite cells appear to establish diverse relationships in these species, which could underlie the different pathological picture. Article in Journal/Newspaper Turbot Wiley Online Library Journal of Fish Diseases 43 3 337 346 |
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English |
description |
Abstract Enteromyxoses are relevant diseases for turbot and gilthead sea bream aquaculture. The myxozoan parasites invade the intestinal mucosa, causing a cachectic syndrome associated with intestinal barrier alteration; nonetheless, their pathological impact is different. Turbot infected by Enteromyxum scophthalmi develop more severe intestinal lesions, reaching mortality rates of 100%, whereas in E. leei ‐infected gilthead sea bream, the disease progresses slowly, and mortality rates are lower. The mechanisms underlying the different pathogenesis are still unclear. We studied the distribution and expression changes of E‐cadherin, a highly conserved protein of the adherens junctions, in the intestine of both species by immunohistochemistry and quantitative PCR, using the same immunohistochemical protocol and common primers. The regular immunostaining pattern observed in control fish turned into markedly irregular in parasitized turbot, showing an intense immunoreaction at the host–parasite interface. Nevertheless, E‐cadherin gene expression was not significantly modulated in this species. On the contrary, no evident changes in the protein distribution were noticed in gilthead sea bream, whereas a significant gene downregulation occurred in advanced infection. The results contribute to the understanding of the different host–parasite interactions in enteromyxoses. Host and parasite cells appear to establish diverse relationships in these species, which could underlie the different pathological picture. |
author2 |
Generalitat Valenciana Horizon 2020 Framework Programme Ministerio de Ciencia e Innovación |
format |
Article in Journal/Newspaper |
author |
Ronza, Paolo Estensoro, Itziar Bermúdez, Roberto Losada, Ana Paula Pérez‐Cordón, Gregorio Pardo, Belén G. Sitjà‐Bobadilla, Ariadna Quiroga, Mª Isabel |
spellingShingle |
Ronza, Paolo Estensoro, Itziar Bermúdez, Roberto Losada, Ana Paula Pérez‐Cordón, Gregorio Pardo, Belén G. Sitjà‐Bobadilla, Ariadna Quiroga, Mª Isabel Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
author_facet |
Ronza, Paolo Estensoro, Itziar Bermúdez, Roberto Losada, Ana Paula Pérez‐Cordón, Gregorio Pardo, Belén G. Sitjà‐Bobadilla, Ariadna Quiroga, Mª Isabel |
author_sort |
Ronza, Paolo |
title |
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
title_short |
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
title_full |
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
title_fullStr |
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
title_full_unstemmed |
Effects of Enteromyxum spp. (Myxozoa) infection in the regulation of intestinal E‐cadherin: Turbot against gilthead sea bream |
title_sort |
effects of enteromyxum spp. (myxozoa) infection in the regulation of intestinal e‐cadherin: turbot against gilthead sea bream |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.13130 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.13130 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Journal of Fish Diseases volume 43, issue 3, page 337-346 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.13130 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
3 |
container_start_page |
337 |
op_container_end_page |
346 |
_version_ |
1810484312276992000 |