Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV
Abstract A novel viral haemorrhagic septicaemia virus ( VHSV ) of genotype IV was isolated from wild lumpfish ( Cyclopterus lumpus ), brought to a land‐based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated...
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crwiley:10.1111/jfd.12910 2024-10-13T14:06:02+00:00 Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Iburg, Tine Moesgaard Olesen, Niels Jørgen 2018 http://dx.doi.org/10.1111/jfd.12910 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12910 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12910 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.12910 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Journal of Fish Diseases volume 42, issue 1, page 47-62 ISSN 0140-7775 1365-2761 journal-article 2018 crwiley https://doi.org/10.1111/jfd.12910 2024-09-17T04:50:36Z Abstract A novel viral haemorrhagic septicaemia virus ( VHSV ) of genotype IV was isolated from wild lumpfish ( Cyclopterus lumpus ), brought to a land‐based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real‐time RT ‐ PCR . Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV . In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re‐isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon ( Salmo salar L.). 10% of the lumpfish shedders and 43%–50% of the cohabiting lumpfish survived after 4 weeks. 80%–92% of the Atlantic salmon survived, but no viral RNA was detected by real‐time RT ‐ PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. Article in Journal/Newspaper Atlantic salmon Iceland Salmo salar Wiley Online Library Journal of Fish Diseases 42 1 47 62 |
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description |
Abstract A novel viral haemorrhagic septicaemia virus ( VHSV ) of genotype IV was isolated from wild lumpfish ( Cyclopterus lumpus ), brought to a land‐based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real‐time RT ‐ PCR . Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV . In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re‐isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon ( Salmo salar L.). 10% of the lumpfish shedders and 43%–50% of the cohabiting lumpfish survived after 4 weeks. 80%–92% of the Atlantic salmon survived, but no viral RNA was detected by real‐time RT ‐ PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters. |
format |
Article in Journal/Newspaper |
author |
Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Iburg, Tine Moesgaard Olesen, Niels Jørgen |
spellingShingle |
Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Iburg, Tine Moesgaard Olesen, Niels Jørgen Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
author_facet |
Guðmundsdóttir, Sigríður Vendramin, Niccoló Cuenca, Argelia Sigurðardóttir, Heiða Kristmundsson, Arni Iburg, Tine Moesgaard Olesen, Niels Jørgen |
author_sort |
Guðmundsdóttir, Sigríður |
title |
Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_short |
Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full |
Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_fullStr |
Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_full_unstemmed |
Outbreak of viral haemorrhagic septicaemia ( VHS) in lumpfish ( Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV |
title_sort |
outbreak of viral haemorrhagic septicaemia ( vhs) in lumpfish ( cyclopterus lumpus) in iceland caused by vhs virus genotype iv |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jfd.12910 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12910 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12910 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.12910 |
genre |
Atlantic salmon Iceland Salmo salar |
genre_facet |
Atlantic salmon Iceland Salmo salar |
op_source |
Journal of Fish Diseases volume 42, issue 1, page 47-62 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1111/jfd.12910 |
container_title |
Journal of Fish Diseases |
container_volume |
42 |
container_issue |
1 |
container_start_page |
47 |
op_container_end_page |
62 |
_version_ |
1812812072344354816 |