A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016
Abstract The acute course of disease in young oysters infected by Os HV ‐1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of a mortality...
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Online Access: | http://dx.doi.org/10.1111/jfd.12883 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12883 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12883 |
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crwiley:10.1111/jfd.12883 2024-09-15T18:29:05+00:00 A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 Burioli, Erika A. V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline 2018 http://dx.doi.org/10.1111/jfd.12883 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12883 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12883 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 41, issue 11, page 1759-1769 ISSN 0140-7775 1365-2761 journal-article 2018 crwiley https://doi.org/10.1111/jfd.12883 2024-08-09T04:26:07Z Abstract The acute course of disease in young oysters infected by Os HV ‐1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of a mortality event that occurred in Normandy (France) in June 2016. The lesions, associated with high Os HV ‐1 DNA quantities, were characterized by severe and diffuse haemocytosis mainly involving blast‐like cells, myocyte degeneration and large, irregularly shaped degenerate eosinophilic cells in the connective tissue. The herpesvirus was identified by negative staining TEM and real‐time PCR . Sequencing of the C region and ORF s 42/43 confirmed that the variants met the definition of Os HV ‐1 μVar. We sequenced 30 other ORF s in twenty Os HV ‐1‐positive individuals and compared them to the μVar specimens isolated between 2009 and 2011. The ORF s encoding putative membrane proteins showed the highest number of variations. Seven different genotypes were identified, confirming the presence of relevant genetic diversity. Phylogenetic analysis provided evidence for a well‐separated μVar new group, with an evolutionary divergence estimated at 0.0013 from the other μVar variants. The geographical distribution of these newly described variants and their effective virulence should be investigated in future. Article in Journal/Newspaper Pacific oyster Wiley Online Library Journal of Fish Diseases 41 11 1759 1769 |
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Wiley Online Library |
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English |
description |
Abstract The acute course of disease in young oysters infected by Os HV ‐1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of a mortality event that occurred in Normandy (France) in June 2016. The lesions, associated with high Os HV ‐1 DNA quantities, were characterized by severe and diffuse haemocytosis mainly involving blast‐like cells, myocyte degeneration and large, irregularly shaped degenerate eosinophilic cells in the connective tissue. The herpesvirus was identified by negative staining TEM and real‐time PCR . Sequencing of the C region and ORF s 42/43 confirmed that the variants met the definition of Os HV ‐1 μVar. We sequenced 30 other ORF s in twenty Os HV ‐1‐positive individuals and compared them to the μVar specimens isolated between 2009 and 2011. The ORF s encoding putative membrane proteins showed the highest number of variations. Seven different genotypes were identified, confirming the presence of relevant genetic diversity. Phylogenetic analysis provided evidence for a well‐separated μVar new group, with an evolutionary divergence estimated at 0.0013 from the other μVar variants. The geographical distribution of these newly described variants and their effective virulence should be investigated in future. |
format |
Article in Journal/Newspaper |
author |
Burioli, Erika A. V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline |
spellingShingle |
Burioli, Erika A. V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
author_facet |
Burioli, Erika A. V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline |
author_sort |
Burioli, Erika A. V. |
title |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_short |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_full |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_fullStr |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_full_unstemmed |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_sort |
novel divergent group of ostreid herpesvirus 1 μvar variants associated with a mortality event in pacific oyster spat in normandy (france) in 2016 |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jfd.12883 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12883 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12883 |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Journal of Fish Diseases volume 41, issue 11, page 1759-1769 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12883 |
container_title |
Journal of Fish Diseases |
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41 |
container_issue |
11 |
container_start_page |
1759 |
op_container_end_page |
1769 |
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1810470494938333184 |