Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua
Abstract In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and in...
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crwiley:10.1111/jfd.12830 2024-04-07T07:50:53+00:00 Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua MacLeod, Michael J. Vo, Nguyen T. K. Mikhaeil, Michael S. Monaghan, S. Richelle Alexander, J. Andrew N. Saran, Mandeep K. Lee, Lucy E. J. Natural Sciences and Engineering Research Council of Canada 2018 http://dx.doi.org/10.1111/jfd.12830 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12830 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12830 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 41, issue 9, page 1359-1372 ISSN 0140-7775 1365-2761 Veterinary (miscellaneous) Aquatic Science journal-article 2018 crwiley https://doi.org/10.1111/jfd.12830 2024-03-08T03:53:15Z Abstract In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial‐scale cultivation approaches. This study details the establishment of a larval cell line ( GML ‐5) from the Atlantic cod ( Gadus morhua ) and its use in the study of microsporidia. GML ‐5 has survived over 100 passages in 8 years of culture. The line remains active and viable between 8 and 21°C in Leibovitz‐15 (L‐15) media with 10% foetal bovine serum and exhibits a myofibroblast phenotype as indicated by immuno‐positive results for vimentin, α‐smooth muscle actin, collagen I and S‐100 proteins, while being desmin‐negative. GML ‐5 supports the infection and development of two microsporidian parasites, an opportunistic generalist ( Anncaliia algerae ) and cod‐specific Loma morhua . Using GML ‐5, spore germination and proliferation of L. morhua was found to require exposure to basic pH and cool incubation temperatures (8°C), in contrast to A. algerae , which required no cultural modifications. Loma morhua ‐associated xenoma‐like structures were observed 2 weeks postexposure. This in vitro infection model may serve as a valuable tool for cod parasitology and aquaculture research. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Loma ENVELOPE(-58.983,-58.983,-62.267,-62.267) Journal of Fish Diseases 41 9 1359 1372 |
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Wiley Online Library |
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language |
English |
topic |
Veterinary (miscellaneous) Aquatic Science |
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Veterinary (miscellaneous) Aquatic Science MacLeod, Michael J. Vo, Nguyen T. K. Mikhaeil, Michael S. Monaghan, S. Richelle Alexander, J. Andrew N. Saran, Mandeep K. Lee, Lucy E. J. Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
topic_facet |
Veterinary (miscellaneous) Aquatic Science |
description |
Abstract In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial‐scale cultivation approaches. This study details the establishment of a larval cell line ( GML ‐5) from the Atlantic cod ( Gadus morhua ) and its use in the study of microsporidia. GML ‐5 has survived over 100 passages in 8 years of culture. The line remains active and viable between 8 and 21°C in Leibovitz‐15 (L‐15) media with 10% foetal bovine serum and exhibits a myofibroblast phenotype as indicated by immuno‐positive results for vimentin, α‐smooth muscle actin, collagen I and S‐100 proteins, while being desmin‐negative. GML ‐5 supports the infection and development of two microsporidian parasites, an opportunistic generalist ( Anncaliia algerae ) and cod‐specific Loma morhua . Using GML ‐5, spore germination and proliferation of L. morhua was found to require exposure to basic pH and cool incubation temperatures (8°C), in contrast to A. algerae , which required no cultural modifications. Loma morhua ‐associated xenoma‐like structures were observed 2 weeks postexposure. This in vitro infection model may serve as a valuable tool for cod parasitology and aquaculture research. |
author2 |
Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
MacLeod, Michael J. Vo, Nguyen T. K. Mikhaeil, Michael S. Monaghan, S. Richelle Alexander, J. Andrew N. Saran, Mandeep K. Lee, Lucy E. J. |
author_facet |
MacLeod, Michael J. Vo, Nguyen T. K. Mikhaeil, Michael S. Monaghan, S. Richelle Alexander, J. Andrew N. Saran, Mandeep K. Lee, Lucy E. J. |
author_sort |
MacLeod, Michael J. |
title |
Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
title_short |
Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
title_full |
Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
title_fullStr |
Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
title_full_unstemmed |
Development of a continuous cell line from larval Atlantic cod ( Gadus morhua ) and its use in the study of the microsporidian, Loma morhua |
title_sort |
development of a continuous cell line from larval atlantic cod ( gadus morhua ) and its use in the study of the microsporidian, loma morhua |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jfd.12830 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12830 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12830 |
long_lat |
ENVELOPE(-58.983,-58.983,-62.267,-62.267) |
geographic |
Loma |
geographic_facet |
Loma |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of Fish Diseases volume 41, issue 9, page 1359-1372 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12830 |
container_title |
Journal of Fish Diseases |
container_volume |
41 |
container_issue |
9 |
container_start_page |
1359 |
op_container_end_page |
1372 |
_version_ |
1795665681167941632 |