The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar
Abstract Piscirickettsiosis is a threatening infectious disease for the salmon industry, due to it being responsible for significant economic losses. The control of outbreaks also poses considerable environmental challenges. Despite Piscirickettsia salmonis having been discovered as the aetiological...
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Online Access: | http://dx.doi.org/10.1111/jfd.12762 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12762 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12762 |
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crwiley:10.1111/jfd.12762 2024-09-15T17:56:28+00:00 The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar Mancilla, M Saavedra, J Grandón, M Tapia, E Navas, E Grothusen, H Bustos, P Corporación de Fomento de la Producción 2017 http://dx.doi.org/10.1111/jfd.12762 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12762 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12762 en eng Wiley http://creativecommons.org/licenses/by-nc/4.0/ Journal of Fish Diseases volume 41, issue 4, page 625-634 ISSN 0140-7775 1365-2761 journal-article 2017 crwiley https://doi.org/10.1111/jfd.12762 2024-07-09T04:15:54Z Abstract Piscirickettsiosis is a threatening infectious disease for the salmon industry, due to it being responsible for significant economic losses. The control of outbreaks also poses considerable environmental challenges. Despite Piscirickettsia salmonis having been discovered as the aetiological agent of the disease more than 25 years ago, its pathogenicity remains poorly understood. Among virulence factors identified so far, type four secretion systems (T4 SS ) seem to play a key role during the infection caused by the bacterium. We report here the genetic manipulation of P . salmonis by means of the transference of plasmid DNA in mating assays. An insertion cassette was engineered for targeting the icmB gene, which encodes a putative T4 SS ‐ ATP ase and is carried by one of the chromosomal T4 SS clusters found within the genome of P. salmonis PM 15972A1, a virulent representative of the EM ‐90‐like strain. The molecular characterization of the resulting mutant strain demonstrated that the insertion interrupted the target gene. Further in vitro testing of the icmB mutant showed a dramatic drop in infectivity as tested in CHSE ‐214 cells, which is in agreement with its attenuated behaviour observed in vivo. Altogether, our results demonstrate that, similar to other facultative intracellular pathogens, P. salmonis ’ virulence relies on an intact T4 SS . Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 41 4 625 634 |
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Wiley Online Library |
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English |
description |
Abstract Piscirickettsiosis is a threatening infectious disease for the salmon industry, due to it being responsible for significant economic losses. The control of outbreaks also poses considerable environmental challenges. Despite Piscirickettsia salmonis having been discovered as the aetiological agent of the disease more than 25 years ago, its pathogenicity remains poorly understood. Among virulence factors identified so far, type four secretion systems (T4 SS ) seem to play a key role during the infection caused by the bacterium. We report here the genetic manipulation of P . salmonis by means of the transference of plasmid DNA in mating assays. An insertion cassette was engineered for targeting the icmB gene, which encodes a putative T4 SS ‐ ATP ase and is carried by one of the chromosomal T4 SS clusters found within the genome of P. salmonis PM 15972A1, a virulent representative of the EM ‐90‐like strain. The molecular characterization of the resulting mutant strain demonstrated that the insertion interrupted the target gene. Further in vitro testing of the icmB mutant showed a dramatic drop in infectivity as tested in CHSE ‐214 cells, which is in agreement with its attenuated behaviour observed in vivo. Altogether, our results demonstrate that, similar to other facultative intracellular pathogens, P. salmonis ’ virulence relies on an intact T4 SS . |
author2 |
Corporación de Fomento de la Producción |
format |
Article in Journal/Newspaper |
author |
Mancilla, M Saavedra, J Grandón, M Tapia, E Navas, E Grothusen, H Bustos, P |
spellingShingle |
Mancilla, M Saavedra, J Grandón, M Tapia, E Navas, E Grothusen, H Bustos, P The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
author_facet |
Mancilla, M Saavedra, J Grandón, M Tapia, E Navas, E Grothusen, H Bustos, P |
author_sort |
Mancilla, M |
title |
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
title_short |
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
title_full |
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
title_fullStr |
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
title_full_unstemmed |
The mutagenesis of a type IV secretion system locus of Piscirickettsia salmonis leads to the attenuation of the pathogen in Atlantic salmon, Salmo salar |
title_sort |
mutagenesis of a type iv secretion system locus of piscirickettsia salmonis leads to the attenuation of the pathogen in atlantic salmon, salmo salar |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/jfd.12762 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12762 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12762 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 41, issue 4, page 625-634 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by-nc/4.0/ |
op_doi |
https://doi.org/10.1111/jfd.12762 |
container_title |
Journal of Fish Diseases |
container_volume |
41 |
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4 |
container_start_page |
625 |
op_container_end_page |
634 |
_version_ |
1810432675965566976 |