Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis
Abstract To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis . Five fish were sampled at post‐exposure days 1, 5, 8...
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Online Access: | http://dx.doi.org/10.1111/jfd.12660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12660 |
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crwiley:10.1111/jfd.12660 2024-09-15T17:56:23+00:00 Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis Díaz, S Rojas, M E Galleguillos, M Maturana, C Smith, P I Cifuentes, F Contreras, I Smith, P A Fondo Nacional de Desarrollo Científico y Tecnológico 2017 http://dx.doi.org/10.1111/jfd.12660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12660 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 40, issue 12, page 1895-1902 ISSN 0140-7775 1365-2761 journal-article 2017 crwiley https://doi.org/10.1111/jfd.12660 2024-07-25T04:18:52Z Abstract To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis . Five fish were sampled at post‐exposure days 1, 5, 8 (preclinical), 20 (clinical) and 40 (post‐clinical period of the disease), and the leucocytes of their coelomic washings were analysed by flow cytometry (using the JC ‐1 cationic dye), TUNEL and cytology to detect apoptotic cells. A selective and temporal pattern of apoptosis modulation by P. salmonis infection was observed. Apoptosis in lymphocytes was not affected, whereas it was inhibited in macrophages but only during the preclinical stage of the induced piscirickettsiosis. Hence, it is postulated that P. salmonis inhibits macrophage apoptosis at the beginning of the disease development to survive, multiply and probably be transported inside these phagocytes; once this process is complete, macrophage apoptosis is no longer inhibited, thus facilitating the exit of the bacteria from the infected cells for continuing their life cycle. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 40 12 1895 1902 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract To improve the understanding of the piscirickettsiosis pathogenesis, the in vivo apoptosis modulation of peritoneal macrophages and lymphocytes was studied in juvenile Salmo salar intraperitoneally injected with Piscirickettsia salmonis . Five fish were sampled at post‐exposure days 1, 5, 8 (preclinical), 20 (clinical) and 40 (post‐clinical period of the disease), and the leucocytes of their coelomic washings were analysed by flow cytometry (using the JC ‐1 cationic dye), TUNEL and cytology to detect apoptotic cells. A selective and temporal pattern of apoptosis modulation by P. salmonis infection was observed. Apoptosis in lymphocytes was not affected, whereas it was inhibited in macrophages but only during the preclinical stage of the induced piscirickettsiosis. Hence, it is postulated that P. salmonis inhibits macrophage apoptosis at the beginning of the disease development to survive, multiply and probably be transported inside these phagocytes; once this process is complete, macrophage apoptosis is no longer inhibited, thus facilitating the exit of the bacteria from the infected cells for continuing their life cycle. |
author2 |
Fondo Nacional de Desarrollo Científico y Tecnológico |
format |
Article in Journal/Newspaper |
author |
Díaz, S Rojas, M E Galleguillos, M Maturana, C Smith, P I Cifuentes, F Contreras, I Smith, P A |
spellingShingle |
Díaz, S Rojas, M E Galleguillos, M Maturana, C Smith, P I Cifuentes, F Contreras, I Smith, P A Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
author_facet |
Díaz, S Rojas, M E Galleguillos, M Maturana, C Smith, P I Cifuentes, F Contreras, I Smith, P A |
author_sort |
Díaz, S |
title |
Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_short |
Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_full |
Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_fullStr |
Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_full_unstemmed |
Apoptosis inhibition of Atlantic salmon ( Salmo salar) peritoneal macrophages by Piscirickettsia salmonis |
title_sort |
apoptosis inhibition of atlantic salmon ( salmo salar) peritoneal macrophages by piscirickettsia salmonis |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/jfd.12660 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12660 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12660 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 40, issue 12, page 1895-1902 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12660 |
container_title |
Journal of Fish Diseases |
container_volume |
40 |
container_issue |
12 |
container_start_page |
1895 |
op_container_end_page |
1902 |
_version_ |
1810432595316441088 |