Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills
Abstract Amoebic gill disease ( AGD ) caused by the ectoparasite Paramoeba perurans affects several cultured marine fish species worldwide. In this study, the morphology and ultrastructure of P. perurans in vitro and in vivo was investigated using scanning and transmission electron microscopy ( SEM...
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crwiley:10.1111/jfd.12444 2024-09-15T17:56:07+00:00 Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills Wiik‐Nielsen, J Mo, T A Kolstad, H Mohammad, S N Hytterød, S Powell, M D Norges Forskningsråd 2016 http://dx.doi.org/10.1111/jfd.12444 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12444 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12444 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 39, issue 9, page 1113-1123 ISSN 0140-7775 1365-2761 journal-article 2016 crwiley https://doi.org/10.1111/jfd.12444 2024-07-25T04:23:09Z Abstract Amoebic gill disease ( AGD ) caused by the ectoparasite Paramoeba perurans affects several cultured marine fish species worldwide. In this study, the morphology and ultrastructure of P. perurans in vitro and in vivo was investigated using scanning and transmission electron microscopy ( SEM and TEM , respectively). Amoebae cultures contained several different morphologies ranging from a distinct rounded cell structure and polymorphic cells with pseudopodia of different lengths and shapes. SEM studies of the gills of AGD ‐affected Atlantic salmon, Salmo salar L., revealed the presence of enlarged swellings in affected gill filaments and fusion of adjacent lamellae. Spherical amoebae appeared to embed within the epithelium, and subsequently leave hemispherical indentations with visible fenestrations in the basolateral surface following their departure. These fenestrated structures corresponded to the presence of pseudopodia which could be seen by TEM to penetrate into the epithelium. The membrane–membrane interface contained an amorphous and slightly fibrous matrix. This suggests the existence of cellular glycocalyces and a role for extracellular products in mediating pathological changes in amoebic gill disease. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 39 9 1113 1123 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Amoebic gill disease ( AGD ) caused by the ectoparasite Paramoeba perurans affects several cultured marine fish species worldwide. In this study, the morphology and ultrastructure of P. perurans in vitro and in vivo was investigated using scanning and transmission electron microscopy ( SEM and TEM , respectively). Amoebae cultures contained several different morphologies ranging from a distinct rounded cell structure and polymorphic cells with pseudopodia of different lengths and shapes. SEM studies of the gills of AGD ‐affected Atlantic salmon, Salmo salar L., revealed the presence of enlarged swellings in affected gill filaments and fusion of adjacent lamellae. Spherical amoebae appeared to embed within the epithelium, and subsequently leave hemispherical indentations with visible fenestrations in the basolateral surface following their departure. These fenestrated structures corresponded to the presence of pseudopodia which could be seen by TEM to penetrate into the epithelium. The membrane–membrane interface contained an amorphous and slightly fibrous matrix. This suggests the existence of cellular glycocalyces and a role for extracellular products in mediating pathological changes in amoebic gill disease. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Wiik‐Nielsen, J Mo, T A Kolstad, H Mohammad, S N Hytterød, S Powell, M D |
spellingShingle |
Wiik‐Nielsen, J Mo, T A Kolstad, H Mohammad, S N Hytterød, S Powell, M D Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
author_facet |
Wiik‐Nielsen, J Mo, T A Kolstad, H Mohammad, S N Hytterød, S Powell, M D |
author_sort |
Wiik‐Nielsen, J |
title |
Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
title_short |
Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
title_full |
Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
title_fullStr |
Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
title_full_unstemmed |
Morphological diversity of Paramoeba perurans trophozoites and their interaction with Atlantic salmon, Salmo salar L., gills |
title_sort |
morphological diversity of paramoeba perurans trophozoites and their interaction with atlantic salmon, salmo salar l., gills |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1111/jfd.12444 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12444 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12444 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 39, issue 9, page 1113-1123 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12444 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
9 |
container_start_page |
1113 |
op_container_end_page |
1123 |
_version_ |
1810432325022908416 |