Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production
Abstract Edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. Insights gained on E. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreak...
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Online Access: | http://dx.doi.org/10.1111/jfd.12389 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12389 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12389 |
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crwiley:10.1111/jfd.12389 2024-09-09T20:12:32+00:00 Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production Castro, N Osorio, C R Buján, N Fuentes, J C Rodríguez, J Romero, M Jiménez, C Toranzo, A E Magariños, B Ministerio de Economía y Competitividad 2015 http://dx.doi.org/10.1111/jfd.12389 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12389 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12389 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 39, issue 5, page 565-576 ISSN 0140-7775 1365-2761 journal-article 2015 crwiley https://doi.org/10.1111/jfd.12389 2024-07-09T04:15:45Z Abstract Edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. Insights gained on E. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. In this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing and siderophore‐mediated ferric uptake systems), in the genome of E. tarda strains isolated from turbot in Europe and their phenotypic traits. A total of 8 genes were tested by PCR for their presence in 73 E. tarda isolates. High homogeneity was observed in the presence/absence pattern of all the strains. Positive results in the amplification of virulence‐related genes were correlated with the detection of chondroitinase activity in agar plates, in vivo AHL production during fish infection and determination of type of siderophore produced by E. tarda . To the best of our knowledge, this is the first study carried out with European strains on potential virulence factors. Furthermore, we demonstrated for the first time that E. tarda produces the siderophore vibrioferrin. Article in Journal/Newspaper Turbot Wiley Online Library Journal of Fish Diseases 39 5 565 576 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. Insights gained on E. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. In this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing and siderophore‐mediated ferric uptake systems), in the genome of E. tarda strains isolated from turbot in Europe and their phenotypic traits. A total of 8 genes were tested by PCR for their presence in 73 E. tarda isolates. High homogeneity was observed in the presence/absence pattern of all the strains. Positive results in the amplification of virulence‐related genes were correlated with the detection of chondroitinase activity in agar plates, in vivo AHL production during fish infection and determination of type of siderophore produced by E. tarda . To the best of our knowledge, this is the first study carried out with European strains on potential virulence factors. Furthermore, we demonstrated for the first time that E. tarda produces the siderophore vibrioferrin. |
author2 |
Ministerio de Economía y Competitividad |
format |
Article in Journal/Newspaper |
author |
Castro, N Osorio, C R Buján, N Fuentes, J C Rodríguez, J Romero, M Jiménez, C Toranzo, A E Magariños, B |
spellingShingle |
Castro, N Osorio, C R Buján, N Fuentes, J C Rodríguez, J Romero, M Jiménez, C Toranzo, A E Magariños, B Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
author_facet |
Castro, N Osorio, C R Buján, N Fuentes, J C Rodríguez, J Romero, M Jiménez, C Toranzo, A E Magariños, B |
author_sort |
Castro, N |
title |
Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
title_short |
Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
title_full |
Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
title_fullStr |
Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
title_full_unstemmed |
Insights into the virulence‐related genes of Edwardsiella tarda isolated from turbot in Europe: genetic homogeneity and evidence for vibrioferrin production |
title_sort |
insights into the virulence‐related genes of edwardsiella tarda isolated from turbot in europe: genetic homogeneity and evidence for vibrioferrin production |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/jfd.12389 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12389 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12389 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Journal of Fish Diseases volume 39, issue 5, page 565-576 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12389 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
5 |
container_start_page |
565 |
op_container_end_page |
576 |
_version_ |
1809947122422775808 |