Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus?
Abstract Viral diseases represent serious challenge in marine farming of Atlantic salmon (Salmo salar L). Pancreas disease ( PD ) caused by a salmonid alphavirus ( SAV ) is by far the most serious in northern Europe. To control PD , it is necessary to identify virus transmission routes. One aspect t...
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crwiley:10.1111/jfd.12382 2024-09-15T17:56:27+00:00 Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? Stene, A Hellebø, A Viljugrein, H Solevåg, S E Devold, M Aspehaug, V The Norwegian Seafood Research Fund - FHF 2015 http://dx.doi.org/10.1111/jfd.12382 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12382 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12382 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 39, issue 5, page 531-537 ISSN 0140-7775 1365-2761 journal-article 2015 crwiley https://doi.org/10.1111/jfd.12382 2024-07-23T04:13:36Z Abstract Viral diseases represent serious challenge in marine farming of Atlantic salmon (Salmo salar L). Pancreas disease ( PD ) caused by a salmonid alphavirus ( SAV ) is by far the most serious in northern Europe. To control PD , it is necessary to identify virus transmission routes. One aspect to consider is whether the virus is transported as free particles or associated with potential vectors. Farmed salmonids have high lipid content in their tissue which may be released into the environment from decomposing dead fish. At the seawater surface, the effects of wind and ocean currents are most prominent. The aim of this study was primarily to identify whether the lipid fraction leaking from dead infected salmon contains SAV . Adipose tissue from dead SAV ‐infected fish from three farming sites was submerged in beakers with sea water in the laboratory and stored at different temperature and time conditions. SAV was identified by real‐time RT ‐ PCR in the lipid fractions accumulating at the water surface in the beakers. SAV ‐ RNA was also present in the sea water. Lipid fractions were transferred to cell culture, and viable SAV was identified. Due to its hydrophobic nature, fat with infective pathogenic virus at the surface may contribute to long‐distance transmission of SAV . Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 39 5 531 537 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Viral diseases represent serious challenge in marine farming of Atlantic salmon (Salmo salar L). Pancreas disease ( PD ) caused by a salmonid alphavirus ( SAV ) is by far the most serious in northern Europe. To control PD , it is necessary to identify virus transmission routes. One aspect to consider is whether the virus is transported as free particles or associated with potential vectors. Farmed salmonids have high lipid content in their tissue which may be released into the environment from decomposing dead fish. At the seawater surface, the effects of wind and ocean currents are most prominent. The aim of this study was primarily to identify whether the lipid fraction leaking from dead infected salmon contains SAV . Adipose tissue from dead SAV ‐infected fish from three farming sites was submerged in beakers with sea water in the laboratory and stored at different temperature and time conditions. SAV was identified by real‐time RT ‐ PCR in the lipid fractions accumulating at the water surface in the beakers. SAV ‐ RNA was also present in the sea water. Lipid fractions were transferred to cell culture, and viable SAV was identified. Due to its hydrophobic nature, fat with infective pathogenic virus at the surface may contribute to long‐distance transmission of SAV . |
author2 |
The Norwegian Seafood Research Fund - FHF |
format |
Article in Journal/Newspaper |
author |
Stene, A Hellebø, A Viljugrein, H Solevåg, S E Devold, M Aspehaug, V |
spellingShingle |
Stene, A Hellebø, A Viljugrein, H Solevåg, S E Devold, M Aspehaug, V Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
author_facet |
Stene, A Hellebø, A Viljugrein, H Solevåg, S E Devold, M Aspehaug, V |
author_sort |
Stene, A |
title |
Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
title_short |
Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
title_full |
Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
title_fullStr |
Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
title_full_unstemmed |
Liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
title_sort |
liquid fat, a potential abiotic vector for horizontal transmission of salmonid alphavirus? |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1111/jfd.12382 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12382 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12382 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 39, issue 5, page 531-537 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12382 |
container_title |
Journal of Fish Diseases |
container_volume |
39 |
container_issue |
5 |
container_start_page |
531 |
op_container_end_page |
537 |
_version_ |
1810432659369754624 |