An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater
Abstract A challenge model for pancreas disease in A tlantic salmon, S almo salar L . fry, was developed comparing two salmonid alphavirus ( SAV ) subtypes: SAV 1 and SAV 5. Viral doses of 3 × 10 5 TCID 50 mL −1 for SAV 1 and 3 × 10 4 for SAV 5 were tested in triplicate tanks, each containing 450 sa...
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Online Access: | http://dx.doi.org/10.1111/jfd.12310 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12310 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12310 |
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crwiley:10.1111/jfd.12310 2024-09-09T19:30:45+00:00 An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater Cano, I Joiner, C Bayley, A Rimmer, G Bateman, K Feist, S W Stone, D Paley, R Cefas DP325 Seedcorn project Defra 2014 http://dx.doi.org/10.1111/jfd.12310 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12310 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12310 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 38, issue 3, page 271-281 ISSN 0140-7775 1365-2761 journal-article 2014 crwiley https://doi.org/10.1111/jfd.12310 2024-07-11T04:38:15Z Abstract A challenge model for pancreas disease in A tlantic salmon, S almo salar L . fry, was developed comparing two salmonid alphavirus ( SAV ) subtypes: SAV 1 and SAV 5. Viral doses of 3 × 10 5 TCID 50 mL −1 for SAV 1 and 3 × 10 4 for SAV 5 were tested in triplicate tanks, each containing 450 salmon fry. Cumulative mortalities of 1.2% were recorded. Titres of virus recovered from the mortalities ranged from 10 2 to 10 7 TCID 50 mL −1 . Fry were sampled at 3, 5 and 7.5 weeks post‐challenge. Sampling after 3 weeks revealed a high prevalence of infection in the absence of clinical signs, and infectious virus was recovered from 80% and 43% of sampled fry infected with SAV 1 and SAV 5, respectively. After 5 weeks pancreas, heart and red skeletal muscle lesions were generally observed, whilst degeneration in white skeletal muscle was observed only in fish infected with SAV 1. In situ hybridisation confirmed the presence of viral genome in infected pancreas, heart and muscle. After 7.5 weeks, infectious virus (both isolates) was recovered from 13.3% of the fish sampled, with a viral titre of 10 2 TCID 50 mL −1 . Clearly, salmon fry are susceptible to SAV infection and pancreas disease. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Almo ENVELOPE(15.306,15.306,66.954,66.954) Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Fish Diseases 38 3 271 281 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A challenge model for pancreas disease in A tlantic salmon, S almo salar L . fry, was developed comparing two salmonid alphavirus ( SAV ) subtypes: SAV 1 and SAV 5. Viral doses of 3 × 10 5 TCID 50 mL −1 for SAV 1 and 3 × 10 4 for SAV 5 were tested in triplicate tanks, each containing 450 salmon fry. Cumulative mortalities of 1.2% were recorded. Titres of virus recovered from the mortalities ranged from 10 2 to 10 7 TCID 50 mL −1 . Fry were sampled at 3, 5 and 7.5 weeks post‐challenge. Sampling after 3 weeks revealed a high prevalence of infection in the absence of clinical signs, and infectious virus was recovered from 80% and 43% of sampled fry infected with SAV 1 and SAV 5, respectively. After 5 weeks pancreas, heart and red skeletal muscle lesions were generally observed, whilst degeneration in white skeletal muscle was observed only in fish infected with SAV 1. In situ hybridisation confirmed the presence of viral genome in infected pancreas, heart and muscle. After 7.5 weeks, infectious virus (both isolates) was recovered from 13.3% of the fish sampled, with a viral titre of 10 2 TCID 50 mL −1 . Clearly, salmon fry are susceptible to SAV infection and pancreas disease. |
author2 |
Cefas DP325 Seedcorn project Defra |
format |
Article in Journal/Newspaper |
author |
Cano, I Joiner, C Bayley, A Rimmer, G Bateman, K Feist, S W Stone, D Paley, R |
spellingShingle |
Cano, I Joiner, C Bayley, A Rimmer, G Bateman, K Feist, S W Stone, D Paley, R An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
author_facet |
Cano, I Joiner, C Bayley, A Rimmer, G Bateman, K Feist, S W Stone, D Paley, R |
author_sort |
Cano, I |
title |
An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
title_short |
An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
title_full |
An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
title_fullStr |
An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
title_full_unstemmed |
An experimental means of transmitting pancreas disease in Atlantic salmon Salmo salar L. fry in freshwater |
title_sort |
experimental means of transmitting pancreas disease in atlantic salmon salmo salar l. fry in freshwater |
publisher |
Wiley |
publishDate |
2014 |
url |
http://dx.doi.org/10.1111/jfd.12310 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12310 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12310 |
long_lat |
ENVELOPE(15.306,15.306,66.954,66.954) ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Almo Sav’ |
geographic_facet |
Almo Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 38, issue 3, page 271-281 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12310 |
container_title |
Journal of Fish Diseases |
container_volume |
38 |
container_issue |
3 |
container_start_page |
271 |
op_container_end_page |
281 |
_version_ |
1809899736925208576 |