In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr
Abstract The effect of serial in vitro subculturing on three pathogenic strains of S aprolegnia parasitica was investigated. The isolates were passed through A tlantic salmon, S almo salar L . parr, and then re‐isolated as single spore colonies. All strains caused infection. The isolate obtained fro...
Published in: | Journal of Fish Diseases |
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crwiley:10.1111/jfd.12175 2024-09-15T17:56:27+00:00 In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr Songe, M M Thoen, E Evensen, Ø Skaar, I 2013 http://dx.doi.org/10.1111/jfd.12175 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12175 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12175 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.12175 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/3.0/ Journal of Fish Diseases volume 37, issue 9, page 825-834 ISSN 0140-7775 1365-2761 journal-article 2013 crwiley https://doi.org/10.1111/jfd.12175 2024-08-13T04:13:47Z Abstract The effect of serial in vitro subculturing on three pathogenic strains of S aprolegnia parasitica was investigated. The isolates were passed through A tlantic salmon, S almo salar L . parr, and then re‐isolated as single spore colonies. All strains caused infection. The isolate obtained from diseased fish served as a virulent reference culture and was designated ‘ AP ’ (‘activated through passage’). Successive subculturing was made by obtaining an inoculum from AP to produce the 2nd subculture and then passaged to the 3rd subculture (from the 2nd), until the 15th passage was obtained. Spores used to produce storage cultures were collected at passages 5, 10 and 15. The different passages of each strain were used to artificially infect A tlantic salmon parr. Morphological characterization of growth patterns was performed to observe differences occurring due to serial in vitro subculturing. Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not. This study is the first to investigate attenuation of virulence in S aprolegnia and whether or not isolates of S . parasitica should be passed through the fish host prior to challenge experiments. It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose–yeast extract. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Diseases 37 9 825 834 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The effect of serial in vitro subculturing on three pathogenic strains of S aprolegnia parasitica was investigated. The isolates were passed through A tlantic salmon, S almo salar L . parr, and then re‐isolated as single spore colonies. All strains caused infection. The isolate obtained from diseased fish served as a virulent reference culture and was designated ‘ AP ’ (‘activated through passage’). Successive subculturing was made by obtaining an inoculum from AP to produce the 2nd subculture and then passaged to the 3rd subculture (from the 2nd), until the 15th passage was obtained. Spores used to produce storage cultures were collected at passages 5, 10 and 15. The different passages of each strain were used to artificially infect A tlantic salmon parr. Morphological characterization of growth patterns was performed to observe differences occurring due to serial in vitro subculturing. Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not. This study is the first to investigate attenuation of virulence in S aprolegnia and whether or not isolates of S . parasitica should be passed through the fish host prior to challenge experiments. It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose–yeast extract. |
format |
Article in Journal/Newspaper |
author |
Songe, M M Thoen, E Evensen, Ø Skaar, I |
spellingShingle |
Songe, M M Thoen, E Evensen, Ø Skaar, I In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
author_facet |
Songe, M M Thoen, E Evensen, Ø Skaar, I |
author_sort |
Songe, M M |
title |
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
title_short |
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
title_full |
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
title_fullStr |
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
title_full_unstemmed |
In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr |
title_sort |
in vitro passages impact on virulence of saprolegnia parasitica to atlantic salmon, salmo salar l. parr |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/jfd.12175 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12175 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12175 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfd.12175 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Diseases volume 37, issue 9, page 825-834 ISSN 0140-7775 1365-2761 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_doi |
https://doi.org/10.1111/jfd.12175 |
container_title |
Journal of Fish Diseases |
container_volume |
37 |
container_issue |
9 |
container_start_page |
825 |
op_container_end_page |
834 |
_version_ |
1810432653252362240 |