Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish
Abstract The enteric myxozoan parasites E nteromyxum leei ( D iamant, L om et D yková) and E nteromyxum scophthalmi P alenzuela, R edondo et Á lvarez‐Pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream ( GSB...
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Online Access: | http://dx.doi.org/10.1111/jfd.12168 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12168 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12168 |
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crwiley:10.1111/jfd.12168 2023-12-03T10:31:28+01:00 Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish Estensoro, I Redondo, M J Álvarez‐Pellitero, P Sitjà‐Bobadilla, A Spanish Ministry of Science and Innovation Generalitat Valenciana PROMETEO ISIC 2013 http://dx.doi.org/10.1111/jfd.12168 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12168 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12168 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 37, issue 9, page 785-796 ISSN 0140-7775 1365-2761 Veterinary (miscellaneous) Aquatic Science journal-article 2013 crwiley https://doi.org/10.1111/jfd.12168 2023-11-09T13:22:20Z Abstract The enteric myxozoan parasites E nteromyxum leei ( D iamant, L om et D yková) and E nteromyxum scophthalmi P alenzuela, R edondo et Á lvarez‐Pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream ( GSB ), S parus aurata L ., and to high mortality rates in turbot, P setta maxima ( L .). The detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific antibodies. Rabbit polyclonal antibodies (p A bs) were raised against E . scophthalmi and E . leei , and direct enzyme‐linked immunosorbent assay ( ELISA ) and immunohistochemistry were used to characterize their sensitivity and specificity. Both p A bs were adsorbed (ap A b) with non‐infected intestines to avoid non‐specific labelling of fish tissues and to improve their specificity. The highest titre obtained in ELISA was 1: 32 000 for ap A b‐ E leei and 1:16 000 for ap A b‐ E scoph. Working dilutions in immunohistochemistry were 1:1000 for ap A b‐ E leei and 1:8000 for ap A b‐ E scoph. Both ap A bs labelled proliferative and sporogonic stages with high specificity. ap A b‐ E scoph was very specific, whereas ap A b‐ E leei cross‐reacted with S phaerospora dicentrarchi S itjà‐ B obadilla et Á lvarez‐ P ellitero and S phaerospora testicularis S itjà‐ B obadilla et Á lvarez‐ P ellitero, suggesting the presence of shared antigens. These p A bs stand as new tools for antigenic characterization and the diagnosis of both E nteromyxum species. Article in Journal/Newspaper Turbot Wiley Online Library (via Crossref) Parus ENVELOPE(3.950,3.950,-71.983,-71.983) Journal of Fish Diseases 37 9 785 796 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Veterinary (miscellaneous) Aquatic Science |
spellingShingle |
Veterinary (miscellaneous) Aquatic Science Estensoro, I Redondo, M J Álvarez‐Pellitero, P Sitjà‐Bobadilla, A Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
topic_facet |
Veterinary (miscellaneous) Aquatic Science |
description |
Abstract The enteric myxozoan parasites E nteromyxum leei ( D iamant, L om et D yková) and E nteromyxum scophthalmi P alenzuela, R edondo et Á lvarez‐Pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream ( GSB ), S parus aurata L ., and to high mortality rates in turbot, P setta maxima ( L .). The detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific antibodies. Rabbit polyclonal antibodies (p A bs) were raised against E . scophthalmi and E . leei , and direct enzyme‐linked immunosorbent assay ( ELISA ) and immunohistochemistry were used to characterize their sensitivity and specificity. Both p A bs were adsorbed (ap A b) with non‐infected intestines to avoid non‐specific labelling of fish tissues and to improve their specificity. The highest titre obtained in ELISA was 1: 32 000 for ap A b‐ E leei and 1:16 000 for ap A b‐ E scoph. Working dilutions in immunohistochemistry were 1:1000 for ap A b‐ E leei and 1:8000 for ap A b‐ E scoph. Both ap A bs labelled proliferative and sporogonic stages with high specificity. ap A b‐ E scoph was very specific, whereas ap A b‐ E leei cross‐reacted with S phaerospora dicentrarchi S itjà‐ B obadilla et Á lvarez‐ P ellitero and S phaerospora testicularis S itjà‐ B obadilla et Á lvarez‐ P ellitero, suggesting the presence of shared antigens. These p A bs stand as new tools for antigenic characterization and the diagnosis of both E nteromyxum species. |
author2 |
Spanish Ministry of Science and Innovation Generalitat Valenciana PROMETEO ISIC |
format |
Article in Journal/Newspaper |
author |
Estensoro, I Redondo, M J Álvarez‐Pellitero, P Sitjà‐Bobadilla, A |
author_facet |
Estensoro, I Redondo, M J Álvarez‐Pellitero, P Sitjà‐Bobadilla, A |
author_sort |
Estensoro, I |
title |
Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
title_short |
Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
title_full |
Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
title_fullStr |
Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
title_full_unstemmed |
Immunohistochemical characterization of polyclonal antibodies against <scp>E</scp>nteromyxum leei and <scp>E</scp>nteromyxum scophthalmi (<scp>M</scp>yxozoa: <scp>M</scp>yxosporea), intestinal parasites of fish |
title_sort |
immunohistochemical characterization of polyclonal antibodies against <scp>e</scp>nteromyxum leei and <scp>e</scp>nteromyxum scophthalmi (<scp>m</scp>yxozoa: <scp>m</scp>yxosporea), intestinal parasites of fish |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/jfd.12168 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12168 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12168 |
long_lat |
ENVELOPE(3.950,3.950,-71.983,-71.983) |
geographic |
Parus |
geographic_facet |
Parus |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Journal of Fish Diseases volume 37, issue 9, page 785-796 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12168 |
container_title |
Journal of Fish Diseases |
container_volume |
37 |
container_issue |
9 |
container_start_page |
785 |
op_container_end_page |
796 |
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1784257755813511168 |