Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis
Abstract Piscirickettsia salmonis is the aetiological agent of piscirickettsiosis, a disease which affects a variety of teleost species and that is particularly severe in salmonid fish. Bacterial‐free supernatants, obtained from cultures of three isolates of P iscirickettsia salmonis, were inoculate...
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crwiley:10.1111/jfd.12019 2024-09-15T17:56:28+00:00 Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis Rojas, M E Galleguillos, M Díaz, S Machuca, A Carbonero, A Smith, P A Grant Fondecyt 2013 http://dx.doi.org/10.1111/jfd.12019 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12019 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12019 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Diseases volume 36, issue 8, page 703-709 ISSN 0140-7775 1365-2761 journal-article 2013 crwiley https://doi.org/10.1111/jfd.12019 2024-07-11T04:35:19Z Abstract Piscirickettsia salmonis is the aetiological agent of piscirickettsiosis, a disease which affects a variety of teleost species and that is particularly severe in salmonid fish. Bacterial‐free supernatants, obtained from cultures of three isolates of P iscirickettsia salmonis, were inoculated in Atlantic salmon, S almo salar L ., and in three continuous cell lines in an effort to determine the presence of secretion of extracellular products ( ECP s) by this microorganism. Although steatosis was found in some liver samples, no mortalities or clinical signs occurred in the inoculated fish. Clear cytotoxicity was observed after inoculation in the cell lines CHSE ‐214 and ASK , derived from salmonid tissues, but not in MDBK , which is of mammalian origin. The degree of cytotoxicity of the ECP s was different among the P . salmonis isolates tested. The isolate that evidenced the highest cytotoxicity in its ECP s exhibited only an intermediate virulence level after challenging fish with bacterial suspensions of the three P . salmonis isolates. Almost complete inhibition of the cytotoxic activity of ECP s was seen after proteinase K treatment, indicating their peptidic nature, and a total preclusion of the cytotoxicity was shown after their incubation at 50 °C for 30 min. Results show that P . salmonis can produce ECP s and at least some of them are thermolabile exotoxins that probably play a role in the pathogenesis of piscirickettsiosis. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Journal of Fish Diseases 36 8 703 709 |
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Abstract Piscirickettsia salmonis is the aetiological agent of piscirickettsiosis, a disease which affects a variety of teleost species and that is particularly severe in salmonid fish. Bacterial‐free supernatants, obtained from cultures of three isolates of P iscirickettsia salmonis, were inoculated in Atlantic salmon, S almo salar L ., and in three continuous cell lines in an effort to determine the presence of secretion of extracellular products ( ECP s) by this microorganism. Although steatosis was found in some liver samples, no mortalities or clinical signs occurred in the inoculated fish. Clear cytotoxicity was observed after inoculation in the cell lines CHSE ‐214 and ASK , derived from salmonid tissues, but not in MDBK , which is of mammalian origin. The degree of cytotoxicity of the ECP s was different among the P . salmonis isolates tested. The isolate that evidenced the highest cytotoxicity in its ECP s exhibited only an intermediate virulence level after challenging fish with bacterial suspensions of the three P . salmonis isolates. Almost complete inhibition of the cytotoxic activity of ECP s was seen after proteinase K treatment, indicating their peptidic nature, and a total preclusion of the cytotoxicity was shown after their incubation at 50 °C for 30 min. Results show that P . salmonis can produce ECP s and at least some of them are thermolabile exotoxins that probably play a role in the pathogenesis of piscirickettsiosis. |
author2 |
Grant Fondecyt |
format |
Article in Journal/Newspaper |
author |
Rojas, M E Galleguillos, M Díaz, S Machuca, A Carbonero, A Smith, P A |
spellingShingle |
Rojas, M E Galleguillos, M Díaz, S Machuca, A Carbonero, A Smith, P A Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
author_facet |
Rojas, M E Galleguillos, M Díaz, S Machuca, A Carbonero, A Smith, P A |
author_sort |
Rojas, M E |
title |
Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
title_short |
Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
title_full |
Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
title_fullStr |
Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
title_full_unstemmed |
Evidence of exotoxin secretion of Piscirickettsia salmonis, the causative agent of piscirickettsiosis |
title_sort |
evidence of exotoxin secretion of piscirickettsia salmonis, the causative agent of piscirickettsiosis |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1111/jfd.12019 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfd.12019 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfd.12019 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases volume 36, issue 8, page 703-709 ISSN 0140-7775 1365-2761 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfd.12019 |
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Journal of Fish Diseases |
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36 |
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8 |
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703 |
op_container_end_page |
709 |
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1810432677027774464 |