Fast and cost‐efficient species identification of Atlantic salmon ( Salmo salar L.), brown trout ( Salmo trutta), and their hybrids using a single SNP marker

Abstract A workflow for developing a cost‐ and time‐efficient, single nucleotide polymorphism (SNP)‐based assay for species and hybrid identification is described. In a reference set ( n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon ( Salmo sal...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Aykanat, Tutku, Balatsou, Athina, Kähkönen, Kirsi, Syrjänen, Jukka T., Janhunen, Matti, Leinonen, Tuomas, Prokkola, Jenni M., Norrgård, Johnny R., Piccolo, John J.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2024
Subjects:
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.1111/jfb.16032
https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.16032
Description
Summary:Abstract A workflow for developing a cost‐ and time‐efficient, single nucleotide polymorphism (SNP)‐based assay for species and hybrid identification is described. In a reference set ( n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon ( Salmo salar L., n = 23) and brown trout ( Salmo trutta , n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field‐collected embryos had 98.1% concordance (155/158) to more expensive and time‐consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.

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