DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight
Abstract The abundances of migratory shark species observed throughout the Mid‐Atlantic Bight (MAB) during productive summer months suggest that this region provides critical habitat and prey resources to these taxa. However, the principal prey assemblages sustaining migratory shark biomass in this...
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Online Access: | http://dx.doi.org/10.1111/jfb.15543 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15543 |
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crwiley:10.1111/jfb.15543 2024-09-15T18:26:23+00:00 DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight Olin, Jill A. Urakawa, Hidetoshi Frisk, Michael G. Newton, Alisa L. Manz, Maria Fogg, Michael McMullen, Colin Crawford, Lisa Shipley, Oliver N. 2023 http://dx.doi.org/10.1111/jfb.15543 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15543 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 103, issue 6, page 1409-1418 ISSN 0022-1112 1095-8649 journal-article 2023 crwiley https://doi.org/10.1111/jfb.15543 2024-08-09T04:25:18Z Abstract The abundances of migratory shark species observed throughout the Mid‐Atlantic Bight (MAB) during productive summer months suggest that this region provides critical habitat and prey resources to these taxa. However, the principal prey assemblages sustaining migratory shark biomass in this region are poorly defined. We applied high‐throughput DNA metabarcoding to shark feces derived from cloacal swabs across nine species of Carcharhinid and Lamnid sharks to (1) quantify the contribution of broad taxa (e.g., invertebrates, fishes) supporting shark biomass during seasonal residency in the MAB and (2) determine whether the species displayed distinct dietary preference indicative of resource partitioning. DNA metabarcoding resulted in high taxonomic (species‐level) resolution of shark diets with actinopterygian and elasmobranch fishes as the dominant prey categories across the species. DNA metabarcoding identified several key prey groups consistent across shark taxa that are likely integral for sustaining their biomass in this region, including Atlantic menhaden ( Brevoortia tyrannus ), Atlantic mackerel ( Scomber scombrus ), and benthic elasmobranchs, including skates. Our results are consistent with previously published stomach content data for the shark species of similar size range in the Northwest Atlantic Ocean, supporting the efficacy of cloacal swab DNA metabarcoding as a minimally invasive diet reconstruction technique. The high reliance of several shark species on Atlantic menhaden could imply wasp‐waist food‐web conditions during the summer months, whereby high abundances of forage fishes sustain a diverse suite of migratory sharks within a complex, seasonal food web. Article in Journal/Newspaper Northwest Atlantic Wiley Online Library Journal of Fish Biology 103 6 1409 1418 |
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English |
description |
Abstract The abundances of migratory shark species observed throughout the Mid‐Atlantic Bight (MAB) during productive summer months suggest that this region provides critical habitat and prey resources to these taxa. However, the principal prey assemblages sustaining migratory shark biomass in this region are poorly defined. We applied high‐throughput DNA metabarcoding to shark feces derived from cloacal swabs across nine species of Carcharhinid and Lamnid sharks to (1) quantify the contribution of broad taxa (e.g., invertebrates, fishes) supporting shark biomass during seasonal residency in the MAB and (2) determine whether the species displayed distinct dietary preference indicative of resource partitioning. DNA metabarcoding resulted in high taxonomic (species‐level) resolution of shark diets with actinopterygian and elasmobranch fishes as the dominant prey categories across the species. DNA metabarcoding identified several key prey groups consistent across shark taxa that are likely integral for sustaining their biomass in this region, including Atlantic menhaden ( Brevoortia tyrannus ), Atlantic mackerel ( Scomber scombrus ), and benthic elasmobranchs, including skates. Our results are consistent with previously published stomach content data for the shark species of similar size range in the Northwest Atlantic Ocean, supporting the efficacy of cloacal swab DNA metabarcoding as a minimally invasive diet reconstruction technique. The high reliance of several shark species on Atlantic menhaden could imply wasp‐waist food‐web conditions during the summer months, whereby high abundances of forage fishes sustain a diverse suite of migratory sharks within a complex, seasonal food web. |
format |
Article in Journal/Newspaper |
author |
Olin, Jill A. Urakawa, Hidetoshi Frisk, Michael G. Newton, Alisa L. Manz, Maria Fogg, Michael McMullen, Colin Crawford, Lisa Shipley, Oliver N. |
spellingShingle |
Olin, Jill A. Urakawa, Hidetoshi Frisk, Michael G. Newton, Alisa L. Manz, Maria Fogg, Michael McMullen, Colin Crawford, Lisa Shipley, Oliver N. DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
author_facet |
Olin, Jill A. Urakawa, Hidetoshi Frisk, Michael G. Newton, Alisa L. Manz, Maria Fogg, Michael McMullen, Colin Crawford, Lisa Shipley, Oliver N. |
author_sort |
Olin, Jill A. |
title |
DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
title_short |
DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
title_full |
DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
title_fullStr |
DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
title_full_unstemmed |
DNA metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the Mid‐Atlantic Bight |
title_sort |
dna metabarcoding of cloacal swabs provides insight into diets of highly migratory sharks in the mid‐atlantic bight |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/jfb.15543 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15543 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_source |
Journal of Fish Biology volume 103, issue 6, page 1409-1418 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfb.15543 |
container_title |
Journal of Fish Biology |
container_volume |
103 |
container_issue |
6 |
container_start_page |
1409 |
op_container_end_page |
1418 |
_version_ |
1810466861734690816 |