Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment
Abstract Polar cod ( Boreogadus saida ) is an important trophic link within Arctic marine food webs and is likely to experience diet shifts in response to climate change. One important tool for assessing organism diet is bulk stable isotope analysis. However, key parameters necessary for interpretin...
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crwiley:10.1111/jfb.15389 2024-03-24T08:59:49+00:00 Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment Ziegler, Amanda Fern Bluhm, Bodil A. Renaud, Paul E. Jørgensen, Lis L. Norges Forskningsråd 2023 http://dx.doi.org/10.1111/jfb.15389 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15389 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Fish Biology volume 102, issue 6, page 1442-1454 ISSN 0022-1112 1095-8649 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2023 crwiley https://doi.org/10.1111/jfb.15389 2024-02-28T02:15:09Z Abstract Polar cod ( Boreogadus saida ) is an important trophic link within Arctic marine food webs and is likely to experience diet shifts in response to climate change. One important tool for assessing organism diet is bulk stable isotope analysis. However, key parameters necessary for interpreting the temporal context of stable isotope values are lacking, especially for Arctic species. This study provides the first experimental determination of isotopic turnover (as half‐life) and trophic discrimination factors (TDFs) of both δ 13 C and δ 15 N in adult polar cod muscle. Using a diet enriched in both 13 C and 15 N, we measured isotopic turnover times of 61 and 49 days for δ 13 C and δ 15 N, respectively, with metabolism accounting for >94% of the total turnover. These half‐life estimates are valid for adult polar cod (>3 years) experiencing little somatic growth. We measured TDFs in our control of 2.6‰ and 3.9‰ for δ 13 C and δ 15 N, respectively, and we conclude that applying the commonly used TDF of ~1‰ for δ 13 C for adult polar cod may lead to misrepresentation of dietary carbon source, while the use of 3.8‰ for δ 15 N is appropriate. Based on these results, we recommend that studies investigating seasonal shifts in the diet of adult polar cod sample at temporal intervals of at least 60 days to account for isotopic turnover in polar cod muscle. Although isotopic equilibrium was reached by the fish in this study, it was at substantially lower isotope values than the diet. Additionally, the use of highly enriched algae in the experimental feed caused very high variability in diet isotope values which precluded accurate calculation of TDFs from the enriched fish. As a result of the challenges faced in this study, we discourage the use of highly enriched diets for similar experiments and provide recommendations to guide the design of future isotopic turnover experiments. Article in Journal/Newspaper Arctic Boreogadus saida Climate change polar cod Wiley Online Library Arctic Journal of Fish Biology 102 6 1442 1454 |
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Wiley Online Library |
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English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
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Aquatic Science Ecology, Evolution, Behavior and Systematics Ziegler, Amanda Fern Bluhm, Bodil A. Renaud, Paul E. Jørgensen, Lis L. Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Abstract Polar cod ( Boreogadus saida ) is an important trophic link within Arctic marine food webs and is likely to experience diet shifts in response to climate change. One important tool for assessing organism diet is bulk stable isotope analysis. However, key parameters necessary for interpreting the temporal context of stable isotope values are lacking, especially for Arctic species. This study provides the first experimental determination of isotopic turnover (as half‐life) and trophic discrimination factors (TDFs) of both δ 13 C and δ 15 N in adult polar cod muscle. Using a diet enriched in both 13 C and 15 N, we measured isotopic turnover times of 61 and 49 days for δ 13 C and δ 15 N, respectively, with metabolism accounting for >94% of the total turnover. These half‐life estimates are valid for adult polar cod (>3 years) experiencing little somatic growth. We measured TDFs in our control of 2.6‰ and 3.9‰ for δ 13 C and δ 15 N, respectively, and we conclude that applying the commonly used TDF of ~1‰ for δ 13 C for adult polar cod may lead to misrepresentation of dietary carbon source, while the use of 3.8‰ for δ 15 N is appropriate. Based on these results, we recommend that studies investigating seasonal shifts in the diet of adult polar cod sample at temporal intervals of at least 60 days to account for isotopic turnover in polar cod muscle. Although isotopic equilibrium was reached by the fish in this study, it was at substantially lower isotope values than the diet. Additionally, the use of highly enriched algae in the experimental feed caused very high variability in diet isotope values which precluded accurate calculation of TDFs from the enriched fish. As a result of the challenges faced in this study, we discourage the use of highly enriched diets for similar experiments and provide recommendations to guide the design of future isotopic turnover experiments. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Ziegler, Amanda Fern Bluhm, Bodil A. Renaud, Paul E. Jørgensen, Lis L. |
author_facet |
Ziegler, Amanda Fern Bluhm, Bodil A. Renaud, Paul E. Jørgensen, Lis L. |
author_sort |
Ziegler, Amanda Fern |
title |
Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
title_short |
Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
title_full |
Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
title_fullStr |
Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
title_full_unstemmed |
Isotopic turnover in polar cod ( Boreogadus saida ) muscle determined through a controlled feeding experiment |
title_sort |
isotopic turnover in polar cod ( boreogadus saida ) muscle determined through a controlled feeding experiment |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/jfb.15389 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15389 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Boreogadus saida Climate change polar cod |
genre_facet |
Arctic Boreogadus saida Climate change polar cod |
op_source |
Journal of Fish Biology volume 102, issue 6, page 1442-1454 ISSN 0022-1112 1095-8649 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/jfb.15389 |
container_title |
Journal of Fish Biology |
container_volume |
102 |
container_issue |
6 |
container_start_page |
1442 |
op_container_end_page |
1454 |
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1794399677880205312 |