Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii )
Abstract Receptor‐interacting serine/threonine‐protein kinase 2 (RIPK2) is an adaptor protein of the pattern recognition receptors NOD1 and NOD2 involved in regulating inflammatory response and resisting pathogenic microbial infection. In this study, Acipenser baerii RIPK2 ( AbRIPK2 ) was identified...
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crwiley:10.1111/jfb.15250 2024-03-24T08:55:30+00:00 Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) Lu, Lu Dai, Shaotong Zhu, Hao Zhang, Xin Li, Zhiqiong Huang, Xiaoli Ouyang, Ping Geng, Yi Chen, Defang Natural Science Foundation of Sichuan Province 2022 http://dx.doi.org/10.1111/jfb.15250 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15250 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.15250 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 102, issue 1, page 178-187 ISSN 0022-1112 1095-8649 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2022 crwiley https://doi.org/10.1111/jfb.15250 2024-02-28T02:17:52Z Abstract Receptor‐interacting serine/threonine‐protein kinase 2 (RIPK2) is an adaptor protein of the pattern recognition receptors NOD1 and NOD2 involved in regulating inflammatory response and resisting pathogenic microbial infection. In this study, Acipenser baerii RIPK2 ( AbRIPK2 ) was identified. The open reading frame of AbRIPK2 was 1815 bp encoding 604 amino acids. AbRIPK2 possessed the typical N‐terminal kinase domain (KD) and C‐terminal caspase recruitment domain (CARD). The phylogenetic tree analysis revealed that AbRIPK2 shared a relatively high identity with bony fish. Real‐time fluorescence quantitative PCR (qRT‐PCR) results indicated that AbRIPK2 was highly expressed in the gill, followed by muscle, liver and heart. AbRIPK2 was significantly induced in the spleen and valvular intestine after Streptococcus iniae and Aeromonas hydrophila infection. AbRIPK2 was significantly upregulated after peptidoglycan (PGN) treatment in the splenic leukocytes. This study indicated that AbRIPK2 played a potential role in resisting the pathogenic infection of Siberian sturgeon by responding to bacteria. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Wiley Online Library Journal of Fish Biology 102 1 178 187 |
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Wiley Online Library |
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English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
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Aquatic Science Ecology, Evolution, Behavior and Systematics Lu, Lu Dai, Shaotong Zhu, Hao Zhang, Xin Li, Zhiqiong Huang, Xiaoli Ouyang, Ping Geng, Yi Chen, Defang Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Abstract Receptor‐interacting serine/threonine‐protein kinase 2 (RIPK2) is an adaptor protein of the pattern recognition receptors NOD1 and NOD2 involved in regulating inflammatory response and resisting pathogenic microbial infection. In this study, Acipenser baerii RIPK2 ( AbRIPK2 ) was identified. The open reading frame of AbRIPK2 was 1815 bp encoding 604 amino acids. AbRIPK2 possessed the typical N‐terminal kinase domain (KD) and C‐terminal caspase recruitment domain (CARD). The phylogenetic tree analysis revealed that AbRIPK2 shared a relatively high identity with bony fish. Real‐time fluorescence quantitative PCR (qRT‐PCR) results indicated that AbRIPK2 was highly expressed in the gill, followed by muscle, liver and heart. AbRIPK2 was significantly induced in the spleen and valvular intestine after Streptococcus iniae and Aeromonas hydrophila infection. AbRIPK2 was significantly upregulated after peptidoglycan (PGN) treatment in the splenic leukocytes. This study indicated that AbRIPK2 played a potential role in resisting the pathogenic infection of Siberian sturgeon by responding to bacteria. |
author2 |
Natural Science Foundation of Sichuan Province |
format |
Article in Journal/Newspaper |
author |
Lu, Lu Dai, Shaotong Zhu, Hao Zhang, Xin Li, Zhiqiong Huang, Xiaoli Ouyang, Ping Geng, Yi Chen, Defang |
author_facet |
Lu, Lu Dai, Shaotong Zhu, Hao Zhang, Xin Li, Zhiqiong Huang, Xiaoli Ouyang, Ping Geng, Yi Chen, Defang |
author_sort |
Lu, Lu |
title |
Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
title_short |
Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
title_full |
Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
title_fullStr |
Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
title_full_unstemmed |
Identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in Siberian sturgeon ( Acipenser baerii ) |
title_sort |
identification and expression profiling of receptor‐interacting serine/threonine‐protein kinase 2 in siberian sturgeon ( acipenser baerii ) |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1111/jfb.15250 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.15250 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.15250 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Journal of Fish Biology volume 102, issue 1, page 178-187 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfb.15250 |
container_title |
Journal of Fish Biology |
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102 |
container_issue |
1 |
container_start_page |
178 |
op_container_end_page |
187 |
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1794400532288241664 |