Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp>
Abstract The white‐blooded Antarctic icefish is the only known vertebrate lacking oxygen‐transporting haemoglobins. Fish skin mucus, as the first line of defence against pathogens, can reflect fish welfare. In this study, we analysed the skin mucus proteome profiles of the two Antarctic fish species...
Published in: | Journal of Fish Biology |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.14908 |
id |
crwiley:10.1111/jfb.14908 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/jfb.14908 2024-04-28T08:00:41+00:00 Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> Huang, Shaojun Jia, Ruonan Hu, Ruiqin Zhai, Wanying Jiang, Shouwen Li, Wenhao Wang, Faxiang Xu, Qianghua National Basic Research Program of China National Natural Science Foundation of China 2021 http://dx.doi.org/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.14908 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 99, issue 6, page 1998-2007 ISSN 0022-1112 1095-8649 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2021 crwiley https://doi.org/10.1111/jfb.14908 2024-04-02T08:47:07Z Abstract The white‐blooded Antarctic icefish is the only known vertebrate lacking oxygen‐transporting haemoglobins. Fish skin mucus, as the first line of defence against pathogens, can reflect fish welfare. In this study, we analysed the skin mucus proteome profiles of the two Antarctic fish species, the white‐blooded Antarctic icefish, Chionodraco hamatus , and the red‐blooded Antarctic fish, Notothenia coriiceps , unfolding the different proteins by liquid chromatography coupled with tandem mass spectrometry isobaric tags for relative and absolute quantitation (iTRAQ) technology. Of the 4444 totally identified proteins, 227 differentially expressed proteins (DEPs) were found in the comparison between C. hamatus and N. coriiceps , of which 121 were upregulated and 106 were downregulated in the icefish. In the Kyoto Encyclopedia of Genes and Genomes pathway annotation, we found two pathways “Legionellosis” and “Complement and coagulation cascades” were significantly enriched, among of which innate immune candidate proteins such as C3, CASP1, ASC, F3 and C9 were significantly upregulated, suggesting their important roles in C. hamatus immune system. Additionally, the DEP protein–protein interaction network analysis and “Response to stress” GO category provided candidate biomarkers for deep understanding of the distinct immune response of the two Antarctic fish underlying the cold adaptation. Article in Journal/Newspaper Antarc* Antarctic Icefish Wiley Online Library Journal of Fish Biology |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Huang, Shaojun Jia, Ruonan Hu, Ruiqin Zhai, Wanying Jiang, Shouwen Li, Wenhao Wang, Faxiang Xu, Qianghua Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Abstract The white‐blooded Antarctic icefish is the only known vertebrate lacking oxygen‐transporting haemoglobins. Fish skin mucus, as the first line of defence against pathogens, can reflect fish welfare. In this study, we analysed the skin mucus proteome profiles of the two Antarctic fish species, the white‐blooded Antarctic icefish, Chionodraco hamatus , and the red‐blooded Antarctic fish, Notothenia coriiceps , unfolding the different proteins by liquid chromatography coupled with tandem mass spectrometry isobaric tags for relative and absolute quantitation (iTRAQ) technology. Of the 4444 totally identified proteins, 227 differentially expressed proteins (DEPs) were found in the comparison between C. hamatus and N. coriiceps , of which 121 were upregulated and 106 were downregulated in the icefish. In the Kyoto Encyclopedia of Genes and Genomes pathway annotation, we found two pathways “Legionellosis” and “Complement and coagulation cascades” were significantly enriched, among of which innate immune candidate proteins such as C3, CASP1, ASC, F3 and C9 were significantly upregulated, suggesting their important roles in C. hamatus immune system. Additionally, the DEP protein–protein interaction network analysis and “Response to stress” GO category provided candidate biomarkers for deep understanding of the distinct immune response of the two Antarctic fish underlying the cold adaptation. |
author2 |
National Basic Research Program of China National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Huang, Shaojun Jia, Ruonan Hu, Ruiqin Zhai, Wanying Jiang, Shouwen Li, Wenhao Wang, Faxiang Xu, Qianghua |
author_facet |
Huang, Shaojun Jia, Ruonan Hu, Ruiqin Zhai, Wanying Jiang, Shouwen Li, Wenhao Wang, Faxiang Xu, Qianghua |
author_sort |
Huang, Shaojun |
title |
Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
title_short |
Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
title_full |
Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
title_fullStr |
Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
title_full_unstemmed |
Specific immunity proteomic profile of the skin mucus of Antarctic fish <scp> Chionodraco hamatus </scp> and <scp> Notothenia coriiceps </scp> |
title_sort |
specific immunity proteomic profile of the skin mucus of antarctic fish <scp> chionodraco hamatus </scp> and <scp> notothenia coriiceps </scp> |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.14908 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.14908 |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_source |
Journal of Fish Biology volume 99, issue 6, page 1998-2007 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfb.14908 |
container_title |
Journal of Fish Biology |
_version_ |
1797572790139748352 |