Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar
Abstract In this study, swim‐tunnel respirometry was performed on Atlantic salmon Salmo salar post‐smolts in a 90 l respirometer on individuals and compared with groups or individuals of similar sizes tested in a 1905 l respirometer, to determine if differences between set‐ups and protocols exist. S...
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crwiley:10.1111/jfb.14087 2024-06-23T07:51:21+00:00 Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar Hvas, Malthe Oppedal, Frode 2019 http://dx.doi.org/10.1111/jfb.14087 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.14087 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.14087 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.14087 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Journal of Fish Biology volume 95, issue 3, page 893-902 ISSN 0022-1112 1095-8649 journal-article 2019 crwiley https://doi.org/10.1111/jfb.14087 2024-06-04T06:49:09Z Abstract In this study, swim‐tunnel respirometry was performed on Atlantic salmon Salmo salar post‐smolts in a 90 l respirometer on individuals and compared with groups or individuals of similar sizes tested in a 1905 l respirometer, to determine if differences between set‐ups and protocols exist. Standard metabolic rate (SMR) derived from the lowest oxygen uptake rate cycles over a 20 h period was statistically similar to SMR derived from back extrapolating to zero swim speed. However, maximum metabolic rate (MMR) estimates varied significantly between swimming at maximum speed, following an exhaustive chase protocol and during confinement stress. Most notably, the mean (±SE) MMR was 511 ± 15 mg O 2 kg −1 h −1 in the swim test which was 52% higher compared with 337 ± 9 mg O 2 kg −1 in the chase protocol, showing that the latter approach causes a substantial underestimation. Performing group respirometry in the larger swim tunnel provided statistically similar estimates of SMR and MMR as for individual fish tested in the smaller tunnel. While we hypothesised a larger swim section and swimming in groups would improve swimming performance, U crit was statistically similar between both set‐ups and statistically similar between swimming alone v . swimming in groups in the larger set‐up, suggesting that this species does not benefit hydrodynamically from swimming in a school in these conditions. Different methods and set‐ups have their own respective limitations and advantages depending on the questions being addressed, the time available, the number of replicates required and if supplementary samplings such as blood or gill tissues are needed. Hence, method choice should be carefully considered when planning experiments and when comparing previous studies. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Journal of Fish Biology |
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Abstract In this study, swim‐tunnel respirometry was performed on Atlantic salmon Salmo salar post‐smolts in a 90 l respirometer on individuals and compared with groups or individuals of similar sizes tested in a 1905 l respirometer, to determine if differences between set‐ups and protocols exist. Standard metabolic rate (SMR) derived from the lowest oxygen uptake rate cycles over a 20 h period was statistically similar to SMR derived from back extrapolating to zero swim speed. However, maximum metabolic rate (MMR) estimates varied significantly between swimming at maximum speed, following an exhaustive chase protocol and during confinement stress. Most notably, the mean (±SE) MMR was 511 ± 15 mg O 2 kg −1 h −1 in the swim test which was 52% higher compared with 337 ± 9 mg O 2 kg −1 in the chase protocol, showing that the latter approach causes a substantial underestimation. Performing group respirometry in the larger swim tunnel provided statistically similar estimates of SMR and MMR as for individual fish tested in the smaller tunnel. While we hypothesised a larger swim section and swimming in groups would improve swimming performance, U crit was statistically similar between both set‐ups and statistically similar between swimming alone v . swimming in groups in the larger set‐up, suggesting that this species does not benefit hydrodynamically from swimming in a school in these conditions. Different methods and set‐ups have their own respective limitations and advantages depending on the questions being addressed, the time available, the number of replicates required and if supplementary samplings such as blood or gill tissues are needed. Hence, method choice should be carefully considered when planning experiments and when comparing previous studies. |
format |
Article in Journal/Newspaper |
author |
Hvas, Malthe Oppedal, Frode |
spellingShingle |
Hvas, Malthe Oppedal, Frode Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
author_facet |
Hvas, Malthe Oppedal, Frode |
author_sort |
Hvas, Malthe |
title |
Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
title_short |
Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
title_full |
Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
title_fullStr |
Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
title_full_unstemmed |
Influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in Atlantic salmon Salmo salar |
title_sort |
influence of experimental set‐up and methodology for measurements of metabolic rates and critical swimming speed in atlantic salmon salmo salar |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1111/jfb.14087 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.14087 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.14087 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/jfb.14087 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Biology volume 95, issue 3, page 893-902 ISSN 0022-1112 1095-8649 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1111/jfb.14087 |
container_title |
Journal of Fish Biology |
_version_ |
1802642425517178880 |