Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon
The efficacy of ploidy analysis for separating progeny of Siberian sturgeon Acipenser baerii after induced gynogenesis was demonstrated using sperm of a paternal species differing in ploidy level from the maternal species. Gynogenesis was induced in tetraploid A. baerii with UV‐C irradiated sperm fr...
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crwiley:10.1111/jfb.13623 2024-09-15T17:34:42+00:00 Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon Lebeda, I. Steinbach, C. Flajšhans, M. Grantová Agentura České Republiky 2018 http://dx.doi.org/10.1111/jfb.13623 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.13623 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.13623 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Fish Biology volume 92, issue 6, page 1819-1831 ISSN 0022-1112 1095-8649 journal-article 2018 crwiley https://doi.org/10.1111/jfb.13623 2024-08-15T04:21:04Z The efficacy of ploidy analysis for separating progeny of Siberian sturgeon Acipenser baerii after induced gynogenesis was demonstrated using sperm of a paternal species differing in ploidy level from the maternal species. Gynogenesis was induced in tetraploid A. baerii with UV‐C irradiated sperm from the diploid sterlet Acipenser ruthenus and vice‐versa . The success of sperm UV irradiation and diploidy restoration by heat‐shock was estimated based on the ploidy level of progeny, confirmed by microsatellite parentage assignment. Hatching rates of interspecific gynogenotes were comparable with rates reported for gynogenesis induction using sperm and eggs of the same species. Juvenile mortality was similar to that observed in the control hybrids. The efficiency and reliability of this method may foster its use for production of gynogenotes in aquaculture, potentially allowing interspecific gynogenesis to replace intraspecific. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Wiley Online Library Journal of Fish Biology 92 6 1819 1831 |
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Wiley Online Library |
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English |
description |
The efficacy of ploidy analysis for separating progeny of Siberian sturgeon Acipenser baerii after induced gynogenesis was demonstrated using sperm of a paternal species differing in ploidy level from the maternal species. Gynogenesis was induced in tetraploid A. baerii with UV‐C irradiated sperm from the diploid sterlet Acipenser ruthenus and vice‐versa . The success of sperm UV irradiation and diploidy restoration by heat‐shock was estimated based on the ploidy level of progeny, confirmed by microsatellite parentage assignment. Hatching rates of interspecific gynogenotes were comparable with rates reported for gynogenesis induction using sperm and eggs of the same species. Juvenile mortality was similar to that observed in the control hybrids. The efficiency and reliability of this method may foster its use for production of gynogenotes in aquaculture, potentially allowing interspecific gynogenesis to replace intraspecific. |
author2 |
Grantová Agentura České Republiky |
format |
Article in Journal/Newspaper |
author |
Lebeda, I. Steinbach, C. Flajšhans, M. |
spellingShingle |
Lebeda, I. Steinbach, C. Flajšhans, M. Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
author_facet |
Lebeda, I. Steinbach, C. Flajšhans, M. |
author_sort |
Lebeda, I. |
title |
Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
title_short |
Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
title_full |
Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
title_fullStr |
Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
title_full_unstemmed |
Flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
title_sort |
flow cytometry for assessing the efficacy of interspecific gynogenesis induction in sturgeon |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jfb.13623 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.13623 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.13623 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Journal of Fish Biology volume 92, issue 6, page 1819-1831 ISSN 0022-1112 1095-8649 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jfb.13623 |
container_title |
Journal of Fish Biology |
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92 |
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6 |
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1819 |
op_container_end_page |
1831 |
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1810289718100754432 |