A toll‐like receptor 3 homologue that is up‐regulated by poly I:C and <scp>DNA</scp> virus in turbot Scophthalmus maximus
In this study, the gene and promoter sequences of turbot Scophthalmus maximus (Sm) toll‐like receptor 3 (Tlr3) were cloned and its mRNA tissue distribution and gene expression in response to polyinosinic:polycytidylic acid (poly I:C) and turbot reddish body iridovirus ( TRBIV ) challenges were studi...
Published in: | Journal of Fish Biology |
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Main Authors: | , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2015
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Subjects: | |
Online Access: | http://dx.doi.org/10.1111/jfb.12559 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.12559 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.12559 |
Summary: | In this study, the gene and promoter sequences of turbot Scophthalmus maximus (Sm) toll‐like receptor 3 (Tlr3) were cloned and its mRNA tissue distribution and gene expression in response to polyinosinic:polycytidylic acid (poly I:C) and turbot reddish body iridovirus ( TRBIV ) challenges were studied in vivo. The smtlr3 gene spans over 4·4 kb with a structure of five exons–four introns and encodes a peptide of 916 amino acids. The putative protein shares the highest sequence identity of 52·8–78·5% with fish Tlr3 and contains a signal peptide sequence, 13 leucine‐rich repeat ( LRR ) motifs, a transmembrane region and a toll/interleukin‐1 receptor ( TIR ) domain. Phylogenetic analysis grouped it with other teleost Tlr3s. A number of transcription factor binding sites were identified in the 1538 bp 5′ flanking region of smtlr3 , including interferon‐stimulated response element ( ISRE ) and those for interferon regulatory factors ( IRF ) and signal transducer and activator of transcriptions ( STATs ) smtlr3 transcripts were expressed ubiquitously with higher levels in the head kidney, heart and digestion organs. They were up‐regulated by both poly I:C and TRBIV in immune and non‐immune organs, but most strongly in the head kidney. Finally, the smtlr3 exhibited a two‐wave induced expression during a five day time course when exposure of S. maximus to poly I:C. These findings provide insights into the role of SmTlr3 in antiviral response. |
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