New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids
Abstract A culture of a unicellular heterotrophic eukaryote was established from pollen‐baited seawater acquired from the nearshore environment in Tromsø, Norway. Light microscopy revealed the production of ectoplasmic nets and reproduction by biflagellated zoospores, as well as binary division. Aft...
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Online Access: | http://dx.doi.org/10.1111/jeu.12494 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeu.12494 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeu.12494 |
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crwiley:10.1111/jeu.12494 2024-09-15T18:39:26+00:00 New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids Hassett, Brandon T. Gradinger, Rolf Norges Forskningsråd 2018 http://dx.doi.org/10.1111/jeu.12494 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeu.12494 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeu.12494 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Eukaryotic Microbiology volume 65, issue 4, page 475-483 ISSN 1066-5234 1550-7408 journal-article 2018 crwiley https://doi.org/10.1111/jeu.12494 2024-08-01T04:19:49Z Abstract A culture of a unicellular heterotrophic eukaryote was established from pollen‐baited seawater acquired from the nearshore environment in Tromsø, Norway. Light microscopy revealed the production of ectoplasmic nets and reproduction by biflagellated zoospores, as well as binary division. After culturing and subsequent nucleotide extraction, database queries of the isolate's 18S small ribosomal subunit coding region identified closest molecular affinity to Aplanochytrium haliotidis , a pathogen of abalone. Testing of phylogenetic hypotheses consistently grouped our unknown isolate and A. haliotidis among the homoplasious thraustochytrids. Transmission electron microscopy revealed complex cell walls comprised of electron‐dense lamella that formed protuberances, some associated with bothrosomes. Co‐culturing experiments with the marine fungus Penicillium brevicompactum revealed prolonged interactions with hyphal strands. Based on the combined information acquired from electron microscopy, life history information, and phylogenetic testing, we describe our unknown isolate as a novel species. To resolve molecular polyphyly within the aplanochytrids, we erect a gen. nov. that circumscribes our novel isolate and the former A. haliotidis within the thraustochytrids. Article in Journal/Newspaper Tromsø Wiley Online Library Journal of Eukaryotic Microbiology 65 4 475 483 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract A culture of a unicellular heterotrophic eukaryote was established from pollen‐baited seawater acquired from the nearshore environment in Tromsø, Norway. Light microscopy revealed the production of ectoplasmic nets and reproduction by biflagellated zoospores, as well as binary division. After culturing and subsequent nucleotide extraction, database queries of the isolate's 18S small ribosomal subunit coding region identified closest molecular affinity to Aplanochytrium haliotidis , a pathogen of abalone. Testing of phylogenetic hypotheses consistently grouped our unknown isolate and A. haliotidis among the homoplasious thraustochytrids. Transmission electron microscopy revealed complex cell walls comprised of electron‐dense lamella that formed protuberances, some associated with bothrosomes. Co‐culturing experiments with the marine fungus Penicillium brevicompactum revealed prolonged interactions with hyphal strands. Based on the combined information acquired from electron microscopy, life history information, and phylogenetic testing, we describe our unknown isolate as a novel species. To resolve molecular polyphyly within the aplanochytrids, we erect a gen. nov. that circumscribes our novel isolate and the former A. haliotidis within the thraustochytrids. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Hassett, Brandon T. Gradinger, Rolf |
spellingShingle |
Hassett, Brandon T. Gradinger, Rolf New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
author_facet |
Hassett, Brandon T. Gradinger, Rolf |
author_sort |
Hassett, Brandon T. |
title |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_short |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_full |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_fullStr |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_full_unstemmed |
New Species of Saprobic Labyrinthulea (=Labyrinthulomycota) and the Erection of a gen. nov. to Resolve Molecular Polyphyly within the Aplanochytrids |
title_sort |
new species of saprobic labyrinthulea (=labyrinthulomycota) and the erection of a gen. nov. to resolve molecular polyphyly within the aplanochytrids |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1111/jeu.12494 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeu.12494 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeu.12494 |
genre |
Tromsø |
genre_facet |
Tromsø |
op_source |
Journal of Eukaryotic Microbiology volume 65, issue 4, page 475-483 ISSN 1066-5234 1550-7408 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jeu.12494 |
container_title |
Journal of Eukaryotic Microbiology |
container_volume |
65 |
container_issue |
4 |
container_start_page |
475 |
op_container_end_page |
483 |
_version_ |
1810483804940271616 |