Morphological and Ribosomal <scp>DNA</scp>‐based Characterization of Six Antarctic Ciliate Morphospecies from the Amundsen Sea with Phylogenetic Analyses

• Published in: Journal of Eukaryotic Microbiology
• Main Authors: Kim, Sun Young, Choi, Joong Ki, Dolan, John R., Shin, Hyoung Chul, Lee, SangHoon, Yang, Eun Jin
• Other Authors: KOPRI
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2013
• Subjects: Microbiology; Amundsen Sea; Antarctic; Antarc*
• Online Access: http://dx.doi.org/10.1111/jeu.12057; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjeu.12057; https://onlinelibrary.wiley.com/doi/pdf/10.1111/jeu.12057

Abstract We characterized six tintinnid ciliates from Antarctic waters using molecular markers and morphological traits: Amphorellopsis quinquealata , Codonellopsis gaussi , Cymatocylis convallaria , Cy. calyciformis , Cy. drygalskii, and Laackmanniella prolongata . The 100% similarity in SSU‐ITS1‐5.8S rDNA ‐ITS2‐partial LSU rDNA sequences among Cy. convallaria , Cy. calyciformis, and Cy. drygalskii is supportive of synonymy. Codonellopsis gaussi and L. prolongata also showed high levels of similarity in SSU rDNA (99.83%) and the D2 domain of LSU rDNA (95.77%), suggesting that they are closely related. Phylogenetic analysis placed Cymatocylis in the Rhabdonellidae, Amphorellopsis in the Tintinnidae and L. prolongata/Co. gaussi within the Dictyocystidae.