Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin
Abstract Background Atopic dermatitis (AD) endotypes differ with ethnicity. We examined the skin microbiota, cytokine‐, and lipid‐profiles in Greenlandic Inuit and Danish children with AD. Methods 25 Inuit children with AD and 25 Inuit control children were clinically examined and compared to previo...
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crwiley:10.1111/jdv.19565 2023-12-03T10:23:40+01:00 Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin Andersson, A. M. Ingham, A. C. Edslev, S. M. Sølberg, J. Skov, L. Koch, A. Ghauharali‐van der Vlugt, K. Stet, F. S. Brüggen, C. M. Jakasa, I. Kezic, S. Thyssen, J. P. 2023 http://dx.doi.org/10.1111/jdv.19565 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jdv.19565 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the European Academy of Dermatology and Venereology ISSN 0926-9959 1468-3083 Infectious Diseases Dermatology journal-article 2023 crwiley https://doi.org/10.1111/jdv.19565 2023-11-09T13:26:52Z Abstract Background Atopic dermatitis (AD) endotypes differ with ethnicity. We examined the skin microbiota, cytokine‐, and lipid‐profiles in Greenlandic Inuit and Danish children with AD. Methods 25 Inuit children with AD and 25 Inuit control children were clinically examined and compared to previously collected data from 25 Danish children with AD. Skin tape strips and skin swabs were collected from lesional and non‐lesional skin. Levels of cutaneous immune biomarkers, free sphingoid bases and their (glycosyl)ceramides were analyzed. Skin swabs were analyzed with 16S rRNA and tuf gene for characterization of bacterial species communities. Results Bacterial β‐diversity was significantly different between Inuit and Danish AD skin, in both lesional ( p <0.001) and non‐lesional ( p <0.001) AD skin, and there was a higher relative abundance of Staphylococcus aureus in Danish compared to Inuit lesional (53% vs. 8%, p <0.01) and non‐lesional skin (55% vs. 5%, p <0.001). Danish AD children had a higher α‐diversity than Inuit children in non‐lesional ( p <0.05) but not in lesional skin. Significantly higher levels of type 2 immunity cytokine interleukin (IL)‐4 ( p <0.05) and IL‐5 ( p <0.01) were identified in Inuit compared to Danish AD children. In contrast, IL‐33 ( p <0.01) was higher in Danish lesional and non‐lesional AD skin. Higher levels of long‐chain glucosylceramide (GlcCER)[S](d26:1) were found in lesional ( p< 0.001) and non‐lesional ( p< 0.001) Inuit skin compared with Danish AD skin. NMF levels were similar in Inuit and Danish AD skin. Conclusion Skin microbiota, cytokine and lipid composition differed significantly between Inuit and Danish children with AD and showed a stronger type 2 immune signature in Inuit children. Article in Journal/Newspaper greenlandic inuit Wiley Online Library (via Crossref) Journal of the European Academy of Dermatology and Venereology |
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Open Polar |
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Wiley Online Library (via Crossref) |
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crwiley |
language |
English |
topic |
Infectious Diseases Dermatology |
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Infectious Diseases Dermatology Andersson, A. M. Ingham, A. C. Edslev, S. M. Sølberg, J. Skov, L. Koch, A. Ghauharali‐van der Vlugt, K. Stet, F. S. Brüggen, C. M. Jakasa, I. Kezic, S. Thyssen, J. P. Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
topic_facet |
Infectious Diseases Dermatology |
description |
Abstract Background Atopic dermatitis (AD) endotypes differ with ethnicity. We examined the skin microbiota, cytokine‐, and lipid‐profiles in Greenlandic Inuit and Danish children with AD. Methods 25 Inuit children with AD and 25 Inuit control children were clinically examined and compared to previously collected data from 25 Danish children with AD. Skin tape strips and skin swabs were collected from lesional and non‐lesional skin. Levels of cutaneous immune biomarkers, free sphingoid bases and their (glycosyl)ceramides were analyzed. Skin swabs were analyzed with 16S rRNA and tuf gene for characterization of bacterial species communities. Results Bacterial β‐diversity was significantly different between Inuit and Danish AD skin, in both lesional ( p <0.001) and non‐lesional ( p <0.001) AD skin, and there was a higher relative abundance of Staphylococcus aureus in Danish compared to Inuit lesional (53% vs. 8%, p <0.01) and non‐lesional skin (55% vs. 5%, p <0.001). Danish AD children had a higher α‐diversity than Inuit children in non‐lesional ( p <0.05) but not in lesional skin. Significantly higher levels of type 2 immunity cytokine interleukin (IL)‐4 ( p <0.05) and IL‐5 ( p <0.01) were identified in Inuit compared to Danish AD children. In contrast, IL‐33 ( p <0.01) was higher in Danish lesional and non‐lesional AD skin. Higher levels of long‐chain glucosylceramide (GlcCER)[S](d26:1) were found in lesional ( p< 0.001) and non‐lesional ( p< 0.001) Inuit skin compared with Danish AD skin. NMF levels were similar in Inuit and Danish AD skin. Conclusion Skin microbiota, cytokine and lipid composition differed significantly between Inuit and Danish children with AD and showed a stronger type 2 immune signature in Inuit children. |
format |
Article in Journal/Newspaper |
author |
Andersson, A. M. Ingham, A. C. Edslev, S. M. Sølberg, J. Skov, L. Koch, A. Ghauharali‐van der Vlugt, K. Stet, F. S. Brüggen, C. M. Jakasa, I. Kezic, S. Thyssen, J. P. |
author_facet |
Andersson, A. M. Ingham, A. C. Edslev, S. M. Sølberg, J. Skov, L. Koch, A. Ghauharali‐van der Vlugt, K. Stet, F. S. Brüggen, C. M. Jakasa, I. Kezic, S. Thyssen, J. P. |
author_sort |
Andersson, A. M. |
title |
Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
title_short |
Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
title_full |
Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
title_fullStr |
Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
title_full_unstemmed |
Ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
title_sort |
ethnic endotypes in pediatric atopic dermatitis depend on immunotype, lipid composition, and microbiota of the skin |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1111/jdv.19565 https://onlinelibrary.wiley.com/doi/pdf/10.1111/jdv.19565 |
genre |
greenlandic inuit |
genre_facet |
greenlandic inuit |
op_source |
Journal of the European Academy of Dermatology and Venereology ISSN 0926-9959 1468-3083 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/jdv.19565 |
container_title |
Journal of the European Academy of Dermatology and Venereology |
_version_ |
1784271894997893120 |