Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard
Background: Abnormal physiological conditions and diseases can change the concentrations of enzymes, metabolites, and minerals in the body. Serum chemistry information may thus be indicative of a specific disease; interpretion of such information requires knowledge of serum chemistry reference inter...
Published in: | Veterinary Clinical Pathology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2006
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1939-165x.2006.tb00114.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1939-165X.2006.tb00114.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1939-165X.2006.tb00114.x |
id |
crwiley:10.1111/j.1939-165x.2006.tb00114.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1939-165x.2006.tb00114.x 2024-06-23T07:51:42+00:00 Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard Tryland,, Morten Thoresen,, Stein Istre Kovacs,, Kit M. Lydersen, Christian 2006 http://dx.doi.org/10.1111/j.1939-165x.2006.tb00114.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1939-165X.2006.tb00114.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1939-165X.2006.tb00114.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Veterinary Clinical Pathology volume 35, issue 2, page 199-203 ISSN 0275-6382 1939-165X journal-article 2006 crwiley https://doi.org/10.1111/j.1939-165x.2006.tb00114.x 2024-06-13T04:25:44Z Background: Abnormal physiological conditions and diseases can change the concentrations of enzymes, metabolites, and minerals in the body. Serum chemistry information may thus be indicative of a specific disease; interpretion of such information requires knowledge of serum chemistry reference intervals from a seemingly healthy population of the species. Objective: The aim of this study was to obtain serum chemistry reference intervals for a population of white whales. Methods: Blood samples were collected from 21 free‐ranging white whales (beluga; Delphinapterus leucas ). The whales were live‐captured in nets during 1996–2001 in Storfjorden, Van Mijenfjorden, and Van Keulenfjorden (Svalbard, Norway). While the whales were briefly physically restrained, blood was collected from the caudal vein into vacuum tubes without anticoagulant. The blood was left to clot for 4–6 hours before serum was obtained by centrifugation. The serum samples were then kept at −20°C until analysis. Enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase [ALP], creatine kinase, lactate dehydrogenase [LDH], amylase, lipase), metabolites (urea, creatinine, bilirubin, cholesterol, triglycerides, nonesterified fatty acids, glucose), and minerals (calcium, phosphate, magnesium, sodium, potassium, chloride) were analyzed in an Advia 1650 System (Bayer, Tarrytown, NY, USA). Cortisol was analyzed in an Immulite One system (Diagnostic Products Corporation, Los Angeles, CA, USA). The major blood proteins (albumin and globulins) were separated by gel electrophoresis in a Beckman Paragon electrophoresis system (Beckman Coulter, Inc., Fullerton, CA, USA). Results: Serum values for all analytes were reported as median and range, and reference intervals were calculated as 10–90th percentiles. Activities of ALP and LDH and cortisol concentration were higher, and protein and bilirubin concentrations were lower compared with those previously reported for white whales from Canada; remaining results were strikingly similar in ... Article in Journal/Newspaper Beluga Beluga* Delphinapterus leucas Storfjorden Svalbard Van Keulenfjorden Van Mijenfjorden Wiley Online Library Svalbard Canada Norway Coulter ENVELOPE(-58.033,-58.033,-83.283,-83.283) Van Mijenfjorden ENVELOPE(14.667,14.667,77.717,77.717) Van Keulenfjorden ENVELOPE(15.489,15.489,77.559,77.559) Veterinary Clinical Pathology 35 2 199 203 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Background: Abnormal physiological conditions and diseases can change the concentrations of enzymes, metabolites, and minerals in the body. Serum chemistry information may thus be indicative of a specific disease; interpretion of such information requires knowledge of serum chemistry reference intervals from a seemingly healthy population of the species. Objective: The aim of this study was to obtain serum chemistry reference intervals for a population of white whales. Methods: Blood samples were collected from 21 free‐ranging white whales (beluga; Delphinapterus leucas ). The whales were live‐captured in nets during 1996–2001 in Storfjorden, Van Mijenfjorden, and Van Keulenfjorden (Svalbard, Norway). While the whales were briefly physically restrained, blood was collected from the caudal vein into vacuum tubes without anticoagulant. The blood was left to clot for 4–6 hours before serum was obtained by centrifugation. The serum samples were then kept at −20°C until analysis. Enzymes (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase [ALP], creatine kinase, lactate dehydrogenase [LDH], amylase, lipase), metabolites (urea, creatinine, bilirubin, cholesterol, triglycerides, nonesterified fatty acids, glucose), and minerals (calcium, phosphate, magnesium, sodium, potassium, chloride) were analyzed in an Advia 1650 System (Bayer, Tarrytown, NY, USA). Cortisol was analyzed in an Immulite One system (Diagnostic Products Corporation, Los Angeles, CA, USA). The major blood proteins (albumin and globulins) were separated by gel electrophoresis in a Beckman Paragon electrophoresis system (Beckman Coulter, Inc., Fullerton, CA, USA). Results: Serum values for all analytes were reported as median and range, and reference intervals were calculated as 10–90th percentiles. Activities of ALP and LDH and cortisol concentration were higher, and protein and bilirubin concentrations were lower compared with those previously reported for white whales from Canada; remaining results were strikingly similar in ... |
format |
Article in Journal/Newspaper |
author |
Tryland,, Morten Thoresen,, Stein Istre Kovacs,, Kit M. Lydersen, Christian |
spellingShingle |
Tryland,, Morten Thoresen,, Stein Istre Kovacs,, Kit M. Lydersen, Christian Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
author_facet |
Tryland,, Morten Thoresen,, Stein Istre Kovacs,, Kit M. Lydersen, Christian |
author_sort |
Tryland,, Morten |
title |
Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
title_short |
Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
title_full |
Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
title_fullStr |
Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
title_full_unstemmed |
Serum chemistry of free‐ranging white whales ( Delphinapterus leucas) in Svalbard |
title_sort |
serum chemistry of free‐ranging white whales ( delphinapterus leucas) in svalbard |
publisher |
Wiley |
publishDate |
2006 |
url |
http://dx.doi.org/10.1111/j.1939-165x.2006.tb00114.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1939-165X.2006.tb00114.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1939-165X.2006.tb00114.x |
long_lat |
ENVELOPE(-58.033,-58.033,-83.283,-83.283) ENVELOPE(14.667,14.667,77.717,77.717) ENVELOPE(15.489,15.489,77.559,77.559) |
geographic |
Svalbard Canada Norway Coulter Van Mijenfjorden Van Keulenfjorden |
geographic_facet |
Svalbard Canada Norway Coulter Van Mijenfjorden Van Keulenfjorden |
genre |
Beluga Beluga* Delphinapterus leucas Storfjorden Svalbard Van Keulenfjorden Van Mijenfjorden |
genre_facet |
Beluga Beluga* Delphinapterus leucas Storfjorden Svalbard Van Keulenfjorden Van Mijenfjorden |
op_source |
Veterinary Clinical Pathology volume 35, issue 2, page 199-203 ISSN 0275-6382 1939-165X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1939-165x.2006.tb00114.x |
container_title |
Veterinary Clinical Pathology |
container_volume |
35 |
container_issue |
2 |
container_start_page |
199 |
op_container_end_page |
203 |
_version_ |
1802642819998810112 |