Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland
Abstract Antibiotics are the foundation of modern medicine, and the sustainability of antibiotic use depends on overcoming the challenge of antibiotic resistance (AR). This may be extremely difficult, given that antibiotic production by environmental microbiota is a natural process and AR is widespr...
Published in: | Journal of Contemporary Water Research & Education |
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crwiley:10.1111/j.1936-704x.2017.03239.x 2024-09-15T17:34:26+00:00 Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland Diaz, Krystalle S. Rich, Virginia I. McLain, Jean E. 2017 http://dx.doi.org/10.1111/j.1936-704x.2017.03239.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1936-704X.2017.03239.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1936-704X.2017.03239.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Contemporary Water Research & Education volume 160, issue 1, page 42-59 ISSN 1936-7031 1936-704X journal-article 2017 crwiley https://doi.org/10.1111/j.1936-704x.2017.03239.x 2024-07-09T04:12:08Z Abstract Antibiotics are the foundation of modern medicine, and the sustainability of antibiotic use depends on overcoming the challenge of antibiotic resistance (AR). This may be extremely difficult, given that antibiotic production by environmental microbiota is a natural process and AR is widespread in nature. AR has been shown to spread readily and quickly, as well as to exist in the absence of anthropogenic forcing (i.e., the application of antibiotics by humans). This study compared a range of approaches to identify AR gene sequences in microbial community genomes in three habitats (Palsa, Bog, and Fen) across a permafrost thaw gradient in Abisko, Sweden. Depending on the search algorithm used, we found a range of 0.72–1.29% AR protein‐coding genes across all habitats, with model‐based search algorithms resulting in greater sensitivity than traditional identity‐based approaches for identifying AR sequences. Though each habitat within the permafrost gradient showed different cell densities, pH, bioavailable carbon, water content, nutrient availability, and other factors that impact microbial life, the difference in overall AR protein abundance between habitats was not significant. Examination of AR at this location will contribute to our understanding of AR presence in natural environments and how AR may be impacted by permafrost thawing related to global warming. Article in Journal/Newspaper Abisko Global warming palsa permafrost Wiley Online Library Journal of Contemporary Water Research & Education 160 1 42 59 |
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Wiley Online Library |
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English |
description |
Abstract Antibiotics are the foundation of modern medicine, and the sustainability of antibiotic use depends on overcoming the challenge of antibiotic resistance (AR). This may be extremely difficult, given that antibiotic production by environmental microbiota is a natural process and AR is widespread in nature. AR has been shown to spread readily and quickly, as well as to exist in the absence of anthropogenic forcing (i.e., the application of antibiotics by humans). This study compared a range of approaches to identify AR gene sequences in microbial community genomes in three habitats (Palsa, Bog, and Fen) across a permafrost thaw gradient in Abisko, Sweden. Depending on the search algorithm used, we found a range of 0.72–1.29% AR protein‐coding genes across all habitats, with model‐based search algorithms resulting in greater sensitivity than traditional identity‐based approaches for identifying AR sequences. Though each habitat within the permafrost gradient showed different cell densities, pH, bioavailable carbon, water content, nutrient availability, and other factors that impact microbial life, the difference in overall AR protein abundance between habitats was not significant. Examination of AR at this location will contribute to our understanding of AR presence in natural environments and how AR may be impacted by permafrost thawing related to global warming. |
format |
Article in Journal/Newspaper |
author |
Diaz, Krystalle S. Rich, Virginia I. McLain, Jean E. |
spellingShingle |
Diaz, Krystalle S. Rich, Virginia I. McLain, Jean E. Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
author_facet |
Diaz, Krystalle S. Rich, Virginia I. McLain, Jean E. |
author_sort |
Diaz, Krystalle S. |
title |
Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
title_short |
Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
title_full |
Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
title_fullStr |
Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
title_full_unstemmed |
Searching for Antibiotic Resistance Genes in a Pristine Arctic Wetland |
title_sort |
searching for antibiotic resistance genes in a pristine arctic wetland |
publisher |
Wiley |
publishDate |
2017 |
url |
http://dx.doi.org/10.1111/j.1936-704x.2017.03239.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1936-704X.2017.03239.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1936-704X.2017.03239.x |
genre |
Abisko Global warming palsa permafrost |
genre_facet |
Abisko Global warming palsa permafrost |
op_source |
Journal of Contemporary Water Research & Education volume 160, issue 1, page 42-59 ISSN 1936-7031 1936-704X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1936-704x.2017.03239.x |
container_title |
Journal of Contemporary Water Research & Education |
container_volume |
160 |
container_issue |
1 |
container_start_page |
42 |
op_container_end_page |
59 |
_version_ |
1810467558975864832 |