X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion
Summary— Digestive mucus of sea‐water adapted eels has been observed and analyzed by the scanning electron microscope (SEM) after rapid freezing at liquid nitrogen temperature followed by freeze‐drying. No chemical procedures were used in this technique. This allowed the maintenance of the mucous co...
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crwiley:10.1111/j.1768-322x.1990.tb00893.x 2023-12-03T10:09:38+01:00 X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion Humbert, Willy Simonneaux, Valérie Kirsch, Raymond 1990 http://dx.doi.org/10.1111/j.1768-322x.1990.tb00893.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1768-322X.1990.tb00893.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1768-322X.1990.tb00893.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biology of the Cell volume 68, issue 1, page 51-55 ISSN 0248-4900 1768-322X Cell Biology General Medicine journal-article 1990 crwiley https://doi.org/10.1111/j.1768-322x.1990.tb00893.x 2023-11-09T14:15:16Z Summary— Digestive mucus of sea‐water adapted eels has been observed and analyzed by the scanning electron microscope (SEM) after rapid freezing at liquid nitrogen temperature followed by freeze‐drying. No chemical procedures were used in this technique. This allowed the maintenance of the mucous coating. Preliminary X‐ray microanalysis carried out on freeze‐fractured and freeze‐dried samples of the oesophagus showed a decrease of K + and an increase of Ca 2+ and Cl − from the basal part of the mucous cell towards its the apical part. This technique has proven to be satisfactory for it prevents translocation and loss of diffusible elements in situ and allows X‐ray microanalysis in the SEM. Article in Journal/Newspaper Anguilla anguilla Wiley Online Library (via Crossref) Biology of the Cell 68 1 51 55 |
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Open Polar |
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Wiley Online Library (via Crossref) |
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crwiley |
language |
English |
topic |
Cell Biology General Medicine |
spellingShingle |
Cell Biology General Medicine Humbert, Willy Simonneaux, Valérie Kirsch, Raymond X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
topic_facet |
Cell Biology General Medicine |
description |
Summary— Digestive mucus of sea‐water adapted eels has been observed and analyzed by the scanning electron microscope (SEM) after rapid freezing at liquid nitrogen temperature followed by freeze‐drying. No chemical procedures were used in this technique. This allowed the maintenance of the mucous coating. Preliminary X‐ray microanalysis carried out on freeze‐fractured and freeze‐dried samples of the oesophagus showed a decrease of K + and an increase of Ca 2+ and Cl − from the basal part of the mucous cell towards its the apical part. This technique has proven to be satisfactory for it prevents translocation and loss of diffusible elements in situ and allows X‐ray microanalysis in the SEM. |
format |
Article in Journal/Newspaper |
author |
Humbert, Willy Simonneaux, Valérie Kirsch, Raymond |
author_facet |
Humbert, Willy Simonneaux, Valérie Kirsch, Raymond |
author_sort |
Humbert, Willy |
title |
X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
title_short |
X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
title_full |
X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
title_fullStr |
X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
title_full_unstemmed |
X‐ray microanalysis of freeze‐dried digestive mucus in Anguilla anguilla L: modifications of ion content during the early steps of secretion |
title_sort |
x‐ray microanalysis of freeze‐dried digestive mucus in anguilla anguilla l: modifications of ion content during the early steps of secretion |
publisher |
Wiley |
publishDate |
1990 |
url |
http://dx.doi.org/10.1111/j.1768-322x.1990.tb00893.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1768-322X.1990.tb00893.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1768-322X.1990.tb00893.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biology of the Cell volume 68, issue 1, page 51-55 ISSN 0248-4900 1768-322X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1768-322x.1990.tb00893.x |
container_title |
Biology of the Cell |
container_volume |
68 |
container_issue |
1 |
container_start_page |
51 |
op_container_end_page |
55 |
_version_ |
1784265996307005440 |