Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry
Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric...
Published in: | Biology of the Cell |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1985
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1768-322x.1985.tb00402.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1768-322X.1985.tb00402.x |
id |
crwiley:10.1111/j.1768-322x.1985.tb00402.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1768-322x.1985.tb00402.x 2023-12-03T10:09:42+01:00 Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry Barni, S. Gerzeli, G. 1985 http://dx.doi.org/10.1111/j.1768-322x.1985.tb00402.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1768-322X.1985.tb00402.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Biology of the Cell volume 54, issue 3, page 261-269 ISSN 0248-4900 1768-322X Cell Biology General Medicine journal-article 1985 crwiley https://doi.org/10.1111/j.1768-322x.1985.tb00402.x 2023-11-09T13:16:49Z Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction) and cytofluoremetric (propidium iodide and Hoechst 33342 fluorochromization) evaluations of DNA staining intensity. In the silver eel, chromatin condensation was more heterogeneous and chromatin also appeared to be looser. The fluorochromes provided information on the different degrees of organization of the chromatin filament, with regard to both primary and higher order DNA structure. In the silver eel, the fine structure showed a higher number of erythrocytes with large euchromatinic areas and the heterochromatinic component was occasionally present in scattered clumps. As for the cytoplasm, in the silver eel a positive correlation could be established between the presence of intact cytoplasmic organelles (mitochondria, Golgi complexes, ribosomes, etc.) and the high fluorescence intensity. This aspect is revealed through the detection of definite membrane components such as glycoconjugates and primary amino groups (PAS reaction and fluorescamine staining, respectively). All these findings allowed us to establish that the transition from the trophic to the premigratory reproductive phase is marked by a higher heterogeneity of the erythrocytes, due to different cell maturation levels. This fact can be ascribed to different metabolic requirements as well as to a more intense erythropoiesis, typical of the most active phase of the eel's life. Article in Journal/Newspaper Anguilla anguilla Wiley Online Library (via Crossref) Biology of the Cell 54 3 261 269 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Cell Biology General Medicine |
spellingShingle |
Cell Biology General Medicine Barni, S. Gerzeli, G. Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
topic_facet |
Cell Biology General Medicine |
description |
Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction) and cytofluoremetric (propidium iodide and Hoechst 33342 fluorochromization) evaluations of DNA staining intensity. In the silver eel, chromatin condensation was more heterogeneous and chromatin also appeared to be looser. The fluorochromes provided information on the different degrees of organization of the chromatin filament, with regard to both primary and higher order DNA structure. In the silver eel, the fine structure showed a higher number of erythrocytes with large euchromatinic areas and the heterochromatinic component was occasionally present in scattered clumps. As for the cytoplasm, in the silver eel a positive correlation could be established between the presence of intact cytoplasmic organelles (mitochondria, Golgi complexes, ribosomes, etc.) and the high fluorescence intensity. This aspect is revealed through the detection of definite membrane components such as glycoconjugates and primary amino groups (PAS reaction and fluorescamine staining, respectively). All these findings allowed us to establish that the transition from the trophic to the premigratory reproductive phase is marked by a higher heterogeneity of the erythrocytes, due to different cell maturation levels. This fact can be ascribed to different metabolic requirements as well as to a more intense erythropoiesis, typical of the most active phase of the eel's life. |
format |
Article in Journal/Newspaper |
author |
Barni, S. Gerzeli, G. |
author_facet |
Barni, S. Gerzeli, G. |
author_sort |
Barni, S. |
title |
Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
title_short |
Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
title_full |
Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
title_fullStr |
Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
title_full_unstemmed |
Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry |
title_sort |
comparative aspects of circulating erythrocytes in the trophic and reproductive phases of european eel. ultrastructure and cytochemistry |
publisher |
Wiley |
publishDate |
1985 |
url |
http://dx.doi.org/10.1111/j.1768-322x.1985.tb00402.x http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1768-322X.1985.tb00402.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Biology of the Cell volume 54, issue 3, page 261-269 ISSN 0248-4900 1768-322X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1768-322x.1985.tb00402.x |
container_title |
Biology of the Cell |
container_volume |
54 |
container_issue |
3 |
container_start_page |
261 |
op_container_end_page |
269 |
_version_ |
1784266803348766720 |