The active methanotrophic community in a wetland from the High Arctic
Summary The dominant terminal process of carbon mineralization in most freshwater wetlands is methanogenesis. With methane being an important greenhouse gas, the predicted warming of the Arctic may provide a positive feedback. However, the amount of methane released to the atmosphere may be controll...
Published in: | Environmental Microbiology Reports |
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crwiley:10.1111/j.1758-2229.2010.00237.x 2024-09-15T18:27:19+00:00 The active methanotrophic community in a wetland from the High Arctic Graef, Christiane Hestnes, Anne Grethe Svenning, Mette Marianne Frenzel, Peter 2011 http://dx.doi.org/10.1111/j.1758-2229.2010.00237.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1758-2229.2010.00237.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1758-2229.2010.00237.x/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Environmental Microbiology Reports volume 3, issue 4, page 466-472 ISSN 1758-2229 1758-2229 journal-article 2011 crwiley https://doi.org/10.1111/j.1758-2229.2010.00237.x 2024-08-27T04:27:20Z Summary The dominant terminal process of carbon mineralization in most freshwater wetlands is methanogenesis. With methane being an important greenhouse gas, the predicted warming of the Arctic may provide a positive feedback. However, the amount of methane released to the atmosphere may be controlled by the activity of methane‐oxidizing bacteria (methanotrophs) living in the oxic surface layer of wetlands. Previously, methanotrophs have been isolated and identified by genetic profiling in High Arctic wetlands showing the presence of only a few genotypes. Two isolates from Solvatnet (Ny‐Ålesund, Spitsbergen; 79°N) are available: Methylobacter tundripaludum (type I) and Methylocystis rosea (type II), raising the question whether the low diversity is a cultivation effect. We have revisited Solvatnet applying stable isotope probing (SIP) with 13 C‐labelled methane. 16S rRNA profiling revealed active type I methanotrophs including M. tundripaludum , while no active type II methanotrophs were identified. These results indicate that the extant M. tundripaludum is an active methane oxidizer at its locus typicus; furthermore, Methylobacter seems to be the dominant active genus. Diversity of methanotrophs was low as compared, e.g. to wetland rice fields in the Mediterranean. This low diversity suggests a high vulnerability of Arctic methanotroph communities, which deserves more attention. Article in Journal/Newspaper Ny Ålesund Ny-Ålesund Spitsbergen Wiley Online Library Environmental Microbiology Reports 3 4 466 472 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Summary The dominant terminal process of carbon mineralization in most freshwater wetlands is methanogenesis. With methane being an important greenhouse gas, the predicted warming of the Arctic may provide a positive feedback. However, the amount of methane released to the atmosphere may be controlled by the activity of methane‐oxidizing bacteria (methanotrophs) living in the oxic surface layer of wetlands. Previously, methanotrophs have been isolated and identified by genetic profiling in High Arctic wetlands showing the presence of only a few genotypes. Two isolates from Solvatnet (Ny‐Ålesund, Spitsbergen; 79°N) are available: Methylobacter tundripaludum (type I) and Methylocystis rosea (type II), raising the question whether the low diversity is a cultivation effect. We have revisited Solvatnet applying stable isotope probing (SIP) with 13 C‐labelled methane. 16S rRNA profiling revealed active type I methanotrophs including M. tundripaludum , while no active type II methanotrophs were identified. These results indicate that the extant M. tundripaludum is an active methane oxidizer at its locus typicus; furthermore, Methylobacter seems to be the dominant active genus. Diversity of methanotrophs was low as compared, e.g. to wetland rice fields in the Mediterranean. This low diversity suggests a high vulnerability of Arctic methanotroph communities, which deserves more attention. |
format |
Article in Journal/Newspaper |
author |
Graef, Christiane Hestnes, Anne Grethe Svenning, Mette Marianne Frenzel, Peter |
spellingShingle |
Graef, Christiane Hestnes, Anne Grethe Svenning, Mette Marianne Frenzel, Peter The active methanotrophic community in a wetland from the High Arctic |
author_facet |
Graef, Christiane Hestnes, Anne Grethe Svenning, Mette Marianne Frenzel, Peter |
author_sort |
Graef, Christiane |
title |
The active methanotrophic community in a wetland from the High Arctic |
title_short |
The active methanotrophic community in a wetland from the High Arctic |
title_full |
The active methanotrophic community in a wetland from the High Arctic |
title_fullStr |
The active methanotrophic community in a wetland from the High Arctic |
title_full_unstemmed |
The active methanotrophic community in a wetland from the High Arctic |
title_sort |
active methanotrophic community in a wetland from the high arctic |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1758-2229.2010.00237.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1758-2229.2010.00237.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1758-2229.2010.00237.x/fullpdf |
genre |
Ny Ålesund Ny-Ålesund Spitsbergen |
genre_facet |
Ny Ålesund Ny-Ålesund Spitsbergen |
op_source |
Environmental Microbiology Reports volume 3, issue 4, page 466-472 ISSN 1758-2229 1758-2229 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1758-2229.2010.00237.x |
container_title |
Environmental Microbiology Reports |
container_volume |
3 |
container_issue |
4 |
container_start_page |
466 |
op_container_end_page |
472 |
_version_ |
1810468531664322560 |