Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study
Owerwintering strategy of the sub‐arctic evergreen Diapensia lapponica has been studied by examining storage substances in the stem cells throughout different seasons. Stem segments were taken from 5 and 20 mm behind the shoot tip, fixed in glutaraldehyde/formaldehyde and embedded in Epon. Semithin...
Published in: | Nordic Journal of Botany |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley
1990
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Online Access: | http://dx.doi.org/10.1111/j.1756-1051.1990.tb02093.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1756-1051.1990.tb02093.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1756-1051.1990.tb02093.x |
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crwiley:10.1111/j.1756-1051.1990.tb02093.x 2023-12-03T10:18:07+01:00 Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study Pihakaski‐Maunsbach, Kaarina Walles, Björn 1990 http://dx.doi.org/10.1111/j.1756-1051.1990.tb02093.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1756-1051.1990.tb02093.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1756-1051.1990.tb02093.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Nordic Journal of Botany volume 10, issue 5, page 493-500 ISSN 0107-055X 1756-1051 Plant Science Ecology, Evolution, Behavior and Systematics journal-article 1990 crwiley https://doi.org/10.1111/j.1756-1051.1990.tb02093.x 2023-11-09T13:32:11Z Owerwintering strategy of the sub‐arctic evergreen Diapensia lapponica has been studied by examining storage substances in the stem cells throughout different seasons. Stem segments were taken from 5 and 20 mm behind the shoot tip, fixed in glutaraldehyde/formaldehyde and embedded in Epon. Semithin sections were stained with Toluidine blue for general observation, Sudan black for lipids, Amido black for proteins and periodic acid‐Schiff s reagent (PAS) for carbohydrates. Lipids occurred abundantly throughout the year and seem to be the main storage substances. They were particularly common in mid‐winter in the lower part of the shoot. The highest lipid content was found in the pith cells and parenchyma cells of the conductive tissues. Large amyloplasts appeared in mid‐summer in higher amounts in the cortex than in the pith of the upper shoot, but vice versa in the lower stem. Starch was almost entirely lacking from November to April. Amido black staining did not indicate presence of protein bodies at any season. The occurrence of lipids and starch grains in the stem of Diapensia is in general similar to the occurrence of these substances in the leaves throughout the annual cycle. Article in Journal/Newspaper Arctic Wiley Online Library (via Crossref) Arctic Nordic Journal of Botany 10 5 493 500 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Plant Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Plant Science Ecology, Evolution, Behavior and Systematics Pihakaski‐Maunsbach, Kaarina Walles, Björn Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
topic_facet |
Plant Science Ecology, Evolution, Behavior and Systematics |
description |
Owerwintering strategy of the sub‐arctic evergreen Diapensia lapponica has been studied by examining storage substances in the stem cells throughout different seasons. Stem segments were taken from 5 and 20 mm behind the shoot tip, fixed in glutaraldehyde/formaldehyde and embedded in Epon. Semithin sections were stained with Toluidine blue for general observation, Sudan black for lipids, Amido black for proteins and periodic acid‐Schiff s reagent (PAS) for carbohydrates. Lipids occurred abundantly throughout the year and seem to be the main storage substances. They were particularly common in mid‐winter in the lower part of the shoot. The highest lipid content was found in the pith cells and parenchyma cells of the conductive tissues. Large amyloplasts appeared in mid‐summer in higher amounts in the cortex than in the pith of the upper shoot, but vice versa in the lower stem. Starch was almost entirely lacking from November to April. Amido black staining did not indicate presence of protein bodies at any season. The occurrence of lipids and starch grains in the stem of Diapensia is in general similar to the occurrence of these substances in the leaves throughout the annual cycle. |
format |
Article in Journal/Newspaper |
author |
Pihakaski‐Maunsbach, Kaarina Walles, Björn |
author_facet |
Pihakaski‐Maunsbach, Kaarina Walles, Björn |
author_sort |
Pihakaski‐Maunsbach, Kaarina |
title |
Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
title_short |
Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
title_full |
Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
title_fullStr |
Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
title_full_unstemmed |
Seasonal variation of storage substances in stem cells of Diapensia lapponica. A light microscopic study |
title_sort |
seasonal variation of storage substances in stem cells of diapensia lapponica. a light microscopic study |
publisher |
Wiley |
publishDate |
1990 |
url |
http://dx.doi.org/10.1111/j.1756-1051.1990.tb02093.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1756-1051.1990.tb02093.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1756-1051.1990.tb02093.x |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Nordic Journal of Botany volume 10, issue 5, page 493-500 ISSN 0107-055X 1756-1051 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1756-1051.1990.tb02093.x |
container_title |
Nordic Journal of Botany |
container_volume |
10 |
container_issue |
5 |
container_start_page |
493 |
op_container_end_page |
500 |
_version_ |
1784265094588268544 |