Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea )
Abstract We report 22 new polymorphic microsatellites for the Ivory gull ( Pagophila eburnea ), and we describe how they can be efficiently co‐amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the n...
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crwiley:10.1111/j.1755-0998.2011.03015.x 2023-12-03T10:23:33+01:00 Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) YANNIC, GLENN SERMIER, ROBERTO AEBISCHER, ADRIAN GAVRILO, MARIA V. GILG, OLIVIER MILJETEIG, CECILIE SABARD, BRIGITTE STRØM, HALLVARD POUIVÉ, EMMANUELLE BROQUET, T. 2011 http://dx.doi.org/10.1111/j.1755-0998.2011.03015.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2011.03015.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2011.03015.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 11, issue 5, page 877-889 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2011 crwiley https://doi.org/10.1111/j.1755-0998.2011.03015.x 2023-11-09T14:03:06Z Abstract We report 22 new polymorphic microsatellites for the Ivory gull ( Pagophila eburnea ), and we describe how they can be efficiently co‐amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland ( n = 21) and Russia (Severnaya Zemlya Archipelago, n = 21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy–Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull. Article in Journal/Newspaper Greenland ivory gull Pagophila eburnea Severnaya Zemlya Wiley Online Library (via Crossref) Greenland Severnaya Zemlya ENVELOPE(98.000,98.000,79.500,79.500) Molecular Ecology Resources 11 5 877 889 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology YANNIC, GLENN SERMIER, ROBERTO AEBISCHER, ADRIAN GAVRILO, MARIA V. GILG, OLIVIER MILJETEIG, CECILIE SABARD, BRIGITTE STRØM, HALLVARD POUIVÉ, EMMANUELLE BROQUET, T. Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract We report 22 new polymorphic microsatellites for the Ivory gull ( Pagophila eburnea ), and we describe how they can be efficiently co‐amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland ( n = 21) and Russia (Severnaya Zemlya Archipelago, n = 21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy–Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull. |
format |
Article in Journal/Newspaper |
author |
YANNIC, GLENN SERMIER, ROBERTO AEBISCHER, ADRIAN GAVRILO, MARIA V. GILG, OLIVIER MILJETEIG, CECILIE SABARD, BRIGITTE STRØM, HALLVARD POUIVÉ, EMMANUELLE BROQUET, T. |
author_facet |
YANNIC, GLENN SERMIER, ROBERTO AEBISCHER, ADRIAN GAVRILO, MARIA V. GILG, OLIVIER MILJETEIG, CECILIE SABARD, BRIGITTE STRØM, HALLVARD POUIVÉ, EMMANUELLE BROQUET, T. |
author_sort |
YANNIC, GLENN |
title |
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
title_short |
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
title_full |
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
title_fullStr |
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
title_full_unstemmed |
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull ( Pagophila eburnea ) |
title_sort |
description of microsatellite markers and genotyping performances using feathers and buccal swabs for the ivory gull ( pagophila eburnea ) |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2011.03015.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2011.03015.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2011.03015.x |
long_lat |
ENVELOPE(98.000,98.000,79.500,79.500) |
geographic |
Greenland Severnaya Zemlya |
geographic_facet |
Greenland Severnaya Zemlya |
genre |
Greenland ivory gull Pagophila eburnea Severnaya Zemlya |
genre_facet |
Greenland ivory gull Pagophila eburnea Severnaya Zemlya |
op_source |
Molecular Ecology Resources volume 11, issue 5, page 877-889 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2011.03015.x |
container_title |
Molecular Ecology Resources |
container_volume |
11 |
container_issue |
5 |
container_start_page |
877 |
op_container_end_page |
889 |
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1784271733778284544 |