A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida )
Abstract Although single‐nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with t...
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crwiley:10.1111/j.1755-0998.2010.02941.x 2023-12-03T10:23:31+01:00 A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) OLSEN, MORTEN TANGE VOLNY, VERONICA HIRSH BÉRUBÉ, MARTINE DIETZ, RUNE LYDERSEN, CHRISTIAN KOVACS, KIT M. DODD, RICHARD S. PALSBøLL, PER J. 2011 http://dx.doi.org/10.1111/j.1755-0998.2010.02941.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2010.02941.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2010.02941.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 11, issue s1, page 9-19 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2011 crwiley https://doi.org/10.1111/j.1755-0998.2010.02941.x 2023-11-09T13:34:51Z Abstract Although single‐nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida , in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re‐sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next‐generation sequencing facilities. Article in Journal/Newspaper Greenland Pusa hispida ringed seal Svalbard Wiley Online Library (via Crossref) Greenland Svalbard Molecular Ecology Resources 11 9 19 |
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Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology OLSEN, MORTEN TANGE VOLNY, VERONICA HIRSH BÉRUBÉ, MARTINE DIETZ, RUNE LYDERSEN, CHRISTIAN KOVACS, KIT M. DODD, RICHARD S. PALSBøLL, PER J. A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract Although single‐nucleotide polymorphisms (SNPs) have become the marker of choice in the field of human genetics, these markers are only slowly emerging in ecological, evolutionary and conservation genetic analyses of nonmodel species. This is partly because of difficulties associated with the discovery and characterization of SNP markers. Herein, we adopted a simple straightforward approach to identifying SNPs, based on screening of a random genomic library. In total, we identified 768 SNPs in the ringed seal, Pusa hispida hispida , in samples from Greenland and Svalbard. Using three seal samples, SNPs were discovered at a rate of one SNP per 402 bp, whereas re‐sequencing of 96 seals increased the density to one SNP per 29 bp. Although applicable to any species of interest, the approach is especially well suited for SNP discovery in nonmodel organisms and is easily implemented in any standard genetics laboratory, circumventing the need for prior genomic data and use of next‐generation sequencing facilities. |
format |
Article in Journal/Newspaper |
author |
OLSEN, MORTEN TANGE VOLNY, VERONICA HIRSH BÉRUBÉ, MARTINE DIETZ, RUNE LYDERSEN, CHRISTIAN KOVACS, KIT M. DODD, RICHARD S. PALSBøLL, PER J. |
author_facet |
OLSEN, MORTEN TANGE VOLNY, VERONICA HIRSH BÉRUBÉ, MARTINE DIETZ, RUNE LYDERSEN, CHRISTIAN KOVACS, KIT M. DODD, RICHARD S. PALSBøLL, PER J. |
author_sort |
OLSEN, MORTEN TANGE |
title |
A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
title_short |
A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
title_full |
A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
title_fullStr |
A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
title_full_unstemmed |
A simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of SNPs in the Artic ringed seal ( Pusa hispida hispida ) |
title_sort |
simple route to single‐nucleotide polymorphisms in a nonmodel species: identification and characterization of snps in the artic ringed seal ( pusa hispida hispida ) |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2010.02941.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2010.02941.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2010.02941.x |
geographic |
Greenland Svalbard |
geographic_facet |
Greenland Svalbard |
genre |
Greenland Pusa hispida ringed seal Svalbard |
genre_facet |
Greenland Pusa hispida ringed seal Svalbard |
op_source |
Molecular Ecology Resources volume 11, issue s1, page 9-19 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2010.02941.x |
container_title |
Molecular Ecology Resources |
container_volume |
11 |
container_start_page |
9 |
op_container_end_page |
19 |
_version_ |
1784271695090024448 |