Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds
Abstract Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox 1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the const...
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crwiley:10.1111/j.1755-0998.2009.02793.x 2024-09-30T14:25:51+00:00 Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds PATEL, SELINA WAUGH, JOHN MILLAR, CRAIG D. LAMBERT, DAVID M. 2010 http://dx.doi.org/10.1111/j.1755-0998.2009.02793.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02793.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02793.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02793.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 10, issue 3, page 431-438 ISSN 1755-098X 1755-0998 journal-article 2010 crwiley https://doi.org/10.1111/j.1755-0998.2009.02793.x 2024-09-05T05:06:48Z Abstract Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox 1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the construction of a DNA barcoding database have already been collected and are available in museums and other institutions. However, many of these specimens are old (>20 years) and are stored as either fixed study skins or dried skeletons. DNA extracted from such historical samples is typically degraded and, generally, only short DNA fragments can be recovered from such specimens making the recovery of the barcoding region as a single fragment difficult. We report two sets of conserved primers that allow the amplification of the entire DNA barcoding region in either three or five overlapping fragments. These primer sets allow the recovery of DNA barcodes from valuable historical specimens that in many cases are unique in that they are unable or unlikely to be collected again. We also report three new primers that in combination allow the effective amplification from modern samples of the entire DNA barcoding region as a single DNA fragment for 17 orders of Southern Hemisphere birds. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic New Zealand Molecular Ecology Resources 10 3 431 438 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox 1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the construction of a DNA barcoding database have already been collected and are available in museums and other institutions. However, many of these specimens are old (>20 years) and are stored as either fixed study skins or dried skeletons. DNA extracted from such historical samples is typically degraded and, generally, only short DNA fragments can be recovered from such specimens making the recovery of the barcoding region as a single fragment difficult. We report two sets of conserved primers that allow the amplification of the entire DNA barcoding region in either three or five overlapping fragments. These primer sets allow the recovery of DNA barcodes from valuable historical specimens that in many cases are unique in that they are unable or unlikely to be collected again. We also report three new primers that in combination allow the effective amplification from modern samples of the entire DNA barcoding region as a single DNA fragment for 17 orders of Southern Hemisphere birds. |
format |
Article in Journal/Newspaper |
author |
PATEL, SELINA WAUGH, JOHN MILLAR, CRAIG D. LAMBERT, DAVID M. |
spellingShingle |
PATEL, SELINA WAUGH, JOHN MILLAR, CRAIG D. LAMBERT, DAVID M. Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
author_facet |
PATEL, SELINA WAUGH, JOHN MILLAR, CRAIG D. LAMBERT, DAVID M. |
author_sort |
PATEL, SELINA |
title |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_short |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_full |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_fullStr |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_full_unstemmed |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_sort |
conserved primers for dna barcoding historical and modern samples from new zealand and antarctic birds |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2009.02793.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02793.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02793.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02793.x |
geographic |
Antarctic New Zealand |
geographic_facet |
Antarctic New Zealand |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Molecular Ecology Resources volume 10, issue 3, page 431-438 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02793.x |
container_title |
Molecular Ecology Resources |
container_volume |
10 |
container_issue |
3 |
container_start_page |
431 |
op_container_end_page |
438 |
_version_ |
1811646469909774336 |