Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar )
Abstract We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were cl...
Published in: | Molecular Ecology Resources |
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Language: | English |
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Online Access: | http://dx.doi.org/10.1111/j.1755-0998.2009.02781.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02781.x |
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crwiley:10.1111/j.1755-0998.2009.02781.x 2023-12-03T10:19:19+01:00 Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) OLAFSSON, KRISTINN HJORLEIFSDOTTIR, SIGRIDUR PAMPOULIE, CHRISTOPHE HREGGVIDSSON, GUDMUNDUR OLI GUDJONSSON, SIGURDUR 2010 http://dx.doi.org/10.1111/j.1755-0998.2009.02781.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02781.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 10, issue 3, page 533-537 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2010 crwiley https://doi.org/10.1111/j.1755-0998.2009.02781.x 2023-11-09T13:47:29Z Abstract We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were clearly typed, and easily interpretable results were obtained. The method was successfully applied in different laboratories, even when different types of DNA polymerase were employed. The method is useful for analysing paternity, population genetics and conservation as well as for selective breeding programmes. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library (via Crossref) Molecular Ecology Resources 10 3 533 537 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology OLAFSSON, KRISTINN HJORLEIFSDOTTIR, SIGRIDUR PAMPOULIE, CHRISTOPHE HREGGVIDSSON, GUDMUNDUR OLI GUDJONSSON, SIGURDUR Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were clearly typed, and easily interpretable results were obtained. The method was successfully applied in different laboratories, even when different types of DNA polymerase were employed. The method is useful for analysing paternity, population genetics and conservation as well as for selective breeding programmes. |
format |
Article in Journal/Newspaper |
author |
OLAFSSON, KRISTINN HJORLEIFSDOTTIR, SIGRIDUR PAMPOULIE, CHRISTOPHE HREGGVIDSSON, GUDMUNDUR OLI GUDJONSSON, SIGURDUR |
author_facet |
OLAFSSON, KRISTINN HJORLEIFSDOTTIR, SIGRIDUR PAMPOULIE, CHRISTOPHE HREGGVIDSSON, GUDMUNDUR OLI GUDJONSSON, SIGURDUR |
author_sort |
OLAFSSON, KRISTINN |
title |
Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
title_short |
Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
title_full |
Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
title_fullStr |
Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
title_full_unstemmed |
Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar ) |
title_sort |
novel set of multiplex assays (salprint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the atlantic salmon ( salmo salar ) |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2009.02781.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02781.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02781.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Molecular Ecology Resources volume 10, issue 3, page 533-537 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02781.x |
container_title |
Molecular Ecology Resources |
container_volume |
10 |
container_issue |
3 |
container_start_page |
533 |
op_container_end_page |
537 |
_version_ |
1784266532591763456 |