Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat
Abstract Pinniped dietary information is crucial for understanding marine ecosystems; however, in the North Atlantic, grey and harbour seals haulout concomitantly and their faeces are visually indistinguishable. Therefore, we developed molecular methods to differentiate the species’ scat. PCR primer...
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crwiley:10.1111/j.1755-0998.2009.02741.x 2023-12-03T10:23:48+01:00 Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat MASLAND, E. D. P. SWEEZY, M. A. ONO, K. A. 2009 http://dx.doi.org/10.1111/j.1755-0998.2009.02741.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02741.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02741.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02741.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 10, issue 1, page 214-217 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2009 crwiley https://doi.org/10.1111/j.1755-0998.2009.02741.x 2023-11-09T13:47:29Z Abstract Pinniped dietary information is crucial for understanding marine ecosystems; however, in the North Atlantic, grey and harbour seals haulout concomitantly and their faeces are visually indistinguishable. Therefore, we developed molecular methods to differentiate the species’ scat. PCR primers were created that amplify a portion of mitochondrial 16S ribosomal DNA in grey and harbour seals. The samples were amplified and the resulting products were digested using the restriction enzyme Tsp509I, producing diagnostic banding patterns. These tools provide a mechanism by which separate dietary analysis can be achieved for grey and harbour seals at mixed haulouts in New England. Article in Journal/Newspaper harbour seal North Atlantic Phoca vitulina Wiley Online Library (via Crossref) Molecular Ecology Resources 10 1 214 217 |
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Open Polar |
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Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology MASLAND, E. D. P. SWEEZY, M. A. ONO, K. A. Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract Pinniped dietary information is crucial for understanding marine ecosystems; however, in the North Atlantic, grey and harbour seals haulout concomitantly and their faeces are visually indistinguishable. Therefore, we developed molecular methods to differentiate the species’ scat. PCR primers were created that amplify a portion of mitochondrial 16S ribosomal DNA in grey and harbour seals. The samples were amplified and the resulting products were digested using the restriction enzyme Tsp509I, producing diagnostic banding patterns. These tools provide a mechanism by which separate dietary analysis can be achieved for grey and harbour seals at mixed haulouts in New England. |
format |
Article in Journal/Newspaper |
author |
MASLAND, E. D. P. SWEEZY, M. A. ONO, K. A. |
author_facet |
MASLAND, E. D. P. SWEEZY, M. A. ONO, K. A. |
author_sort |
MASLAND, E. D. P. |
title |
Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
title_short |
Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
title_full |
Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
title_fullStr |
Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
title_full_unstemmed |
Molecular methods for differentiating grey seal ( Halichoerus grypus ) and harbour seal ( Phoca vitulina ) scat |
title_sort |
molecular methods for differentiating grey seal ( halichoerus grypus ) and harbour seal ( phoca vitulina ) scat |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2009.02741.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02741.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02741.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02741.x |
genre |
harbour seal North Atlantic Phoca vitulina |
genre_facet |
harbour seal North Atlantic Phoca vitulina |
op_source |
Molecular Ecology Resources volume 10, issue 1, page 214-217 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02741.x |
container_title |
Molecular Ecology Resources |
container_volume |
10 |
container_issue |
1 |
container_start_page |
214 |
op_container_end_page |
217 |
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1784272061659611136 |