A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago
Abstract Previous research on barcoding sedges ( Carex ) suggested that basic searches within a global barcoding database would probably not resolve more than 60% of the world’s some 2000 species. In this study, we take an alternative approach and explore the performance of plant DNA barcoding in th...
Published in: | Molecular Ecology Resources |
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Online Access: | http://dx.doi.org/10.1111/j.1755-0998.2009.02725.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02725.x |
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crwiley:10.1111/j.1755-0998.2009.02725.x 2024-06-23T07:48:50+00:00 A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago CLERC‐BLAIN, JESSICA LE STARR, JULIAN R. BULL, ROGER D. SAARELA, JEFFERY M. 2009 http://dx.doi.org/10.1111/j.1755-0998.2009.02725.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02725.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 10, issue 1, page 69-91 ISSN 1755-098X 1755-0998 journal-article 2009 crwiley https://doi.org/10.1111/j.1755-0998.2009.02725.x 2024-06-11T04:47:52Z Abstract Previous research on barcoding sedges ( Carex ) suggested that basic searches within a global barcoding database would probably not resolve more than 60% of the world’s some 2000 species. In this study, we take an alternative approach and explore the performance of plant DNA barcoding in the Carex lineage from an explicitly regional perspective. We characterize the utility of a subset of the proposed protein‐coding and noncoding plastid barcoding regions ( matK , rpoB , rpoC1 , rbcL , atpF‐atpH , psbK‐psbI ) for distinguishing species of Carex and Kobresia in the Canadian Arctic Archipelago, a clearly defined eco‐geographical region representing 1% of the Earth’s landmass. Our results show that matK resolves the greatest number of species of any single‐locus (95%), and when combined in a two‐locus barcode, it provides 100% species resolution in all but one combination ( matK + atpFH ) during unweighted pair‐group method with arithmetic mean averages (UPGMA) analyses. Noncoding regions were equally or more variable than matK , but as single markers they resolve substantially fewer taxa than matK alone. When difficulties with sequencing and alignment due to microstructural variation in noncoding regions are also considered, our results support other studies in suggesting that protein‐coding regions are more practical as barcoding markers. Plastid DNA barcodes are an effective identification tool for species of Carex and Kobresia in the Canadian Arctic Archipelago, a region where the number of co‐existing closely related species is limited. We suggest that if a regional approach to plant DNA barcoding was applied on a global scale, it could provide a solution to the generally poor species resolution seen in previous barcoding studies. Article in Journal/Newspaper Arctic Archipelago Arctic Canadian Arctic Archipelago Wiley Online Library Arctic Canadian Arctic Archipelago Molecular Ecology Resources 10 1 69 91 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Previous research on barcoding sedges ( Carex ) suggested that basic searches within a global barcoding database would probably not resolve more than 60% of the world’s some 2000 species. In this study, we take an alternative approach and explore the performance of plant DNA barcoding in the Carex lineage from an explicitly regional perspective. We characterize the utility of a subset of the proposed protein‐coding and noncoding plastid barcoding regions ( matK , rpoB , rpoC1 , rbcL , atpF‐atpH , psbK‐psbI ) for distinguishing species of Carex and Kobresia in the Canadian Arctic Archipelago, a clearly defined eco‐geographical region representing 1% of the Earth’s landmass. Our results show that matK resolves the greatest number of species of any single‐locus (95%), and when combined in a two‐locus barcode, it provides 100% species resolution in all but one combination ( matK + atpFH ) during unweighted pair‐group method with arithmetic mean averages (UPGMA) analyses. Noncoding regions were equally or more variable than matK , but as single markers they resolve substantially fewer taxa than matK alone. When difficulties with sequencing and alignment due to microstructural variation in noncoding regions are also considered, our results support other studies in suggesting that protein‐coding regions are more practical as barcoding markers. Plastid DNA barcodes are an effective identification tool for species of Carex and Kobresia in the Canadian Arctic Archipelago, a region where the number of co‐existing closely related species is limited. We suggest that if a regional approach to plant DNA barcoding was applied on a global scale, it could provide a solution to the generally poor species resolution seen in previous barcoding studies. |
format |
Article in Journal/Newspaper |
author |
CLERC‐BLAIN, JESSICA LE STARR, JULIAN R. BULL, ROGER D. SAARELA, JEFFERY M. |
spellingShingle |
CLERC‐BLAIN, JESSICA LE STARR, JULIAN R. BULL, ROGER D. SAARELA, JEFFERY M. A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
author_facet |
CLERC‐BLAIN, JESSICA LE STARR, JULIAN R. BULL, ROGER D. SAARELA, JEFFERY M. |
author_sort |
CLERC‐BLAIN, JESSICA LE |
title |
A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
title_short |
A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
title_full |
A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
title_fullStr |
A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
title_full_unstemmed |
A regional approach to plant DNA barcoding provides high species resolution of sedges ( Carex and Kobresia, Cyperaceae) in the Canadian Arctic Archipelago |
title_sort |
regional approach to plant dna barcoding provides high species resolution of sedges ( carex and kobresia, cyperaceae) in the canadian arctic archipelago |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2009.02725.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02725.x https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02725.x |
geographic |
Arctic Canadian Arctic Archipelago |
geographic_facet |
Arctic Canadian Arctic Archipelago |
genre |
Arctic Archipelago Arctic Canadian Arctic Archipelago |
genre_facet |
Arctic Archipelago Arctic Canadian Arctic Archipelago |
op_source |
Molecular Ecology Resources volume 10, issue 1, page 69-91 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02725.x |
container_title |
Molecular Ecology Resources |
container_volume |
10 |
container_issue |
1 |
container_start_page |
69 |
op_container_end_page |
91 |
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1802639174499565568 |