Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas
Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels...
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Online Access: | http://dx.doi.org/10.1111/j.1755-0998.2009.02525.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02525.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02525.x |
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crwiley:10.1111/j.1755-0998.2009.02525.x 2023-12-03T10:21:24+01:00 Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas SAUVAGE, C. BOUDRY, P. LAPÈGUE, S. 2009 http://dx.doi.org/10.1111/j.1755-0998.2009.02525.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02525.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02525.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 9, issue 3, page 853-855 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2009 crwiley https://doi.org/10.1111/j.1755-0998.2009.02525.x 2023-11-09T13:28:53Z Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library (via Crossref) Pacific Molecular Ecology Resources 9 3 853 855 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology SAUVAGE, C. BOUDRY, P. LAPÈGUE, S. Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance. |
format |
Article in Journal/Newspaper |
author |
SAUVAGE, C. BOUDRY, P. LAPÈGUE, S. |
author_facet |
SAUVAGE, C. BOUDRY, P. LAPÈGUE, S. |
author_sort |
SAUVAGE, C. |
title |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_short |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_full |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_fullStr |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas |
title_sort |
identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the pacific oyster crassostrea gigas |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2009.02525.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02525.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02525.x |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Molecular Ecology Resources volume 9, issue 3, page 853-855 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02525.x |
container_title |
Molecular Ecology Resources |
container_volume |
9 |
container_issue |
3 |
container_start_page |
853 |
op_container_end_page |
855 |
_version_ |
1784269090920071168 |