Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence tags in the Pacific oyster Crassostrea gigas

Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: SAUVAGE, C., BOUDRY, P., LAPÈGUE, S.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2009
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
Pacific
Crassostrea gigas
Pacific oyster
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2009.02525.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2009.02525.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2009.02525.x
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Summary:Abstract We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes‐Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.

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