Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla )
Abstract A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty‐eight microsatellites were...
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crwiley:10.1111/j.1755-0998.2008.02419.x 2023-12-03T10:09:33+01:00 Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) PUJOLAR, J. M. MAES, G. E. VAN HOUDT, J. K. J. ZANE, L. 2009 http://dx.doi.org/10.1111/j.1755-0998.2008.02419.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02419.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02419.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 9, issue 1, page 233-235 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2009 crwiley https://doi.org/10.1111/j.1755-0998.2008.02419.x 2023-11-09T13:38:44Z Abstract A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty‐eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel ( Anguilla rostrata ). Article in Journal/Newspaper Anguilla anguilla Wiley Online Library (via Crossref) Molecular Ecology Resources 9 1 233 235 |
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Open Polar |
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Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology PUJOLAR, J. M. MAES, G. E. VAN HOUDT, J. K. J. ZANE, L. Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract A European eel ( Anguilla anguilla ) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty‐eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel ( Anguilla rostrata ). |
format |
Article in Journal/Newspaper |
author |
PUJOLAR, J. M. MAES, G. E. VAN HOUDT, J. K. J. ZANE, L. |
author_facet |
PUJOLAR, J. M. MAES, G. E. VAN HOUDT, J. K. J. ZANE, L. |
author_sort |
PUJOLAR, J. M. |
title |
Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_short |
Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_full |
Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_fullStr |
Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_full_unstemmed |
Isolation and characterization of expressed sequence tag‐linked microsatellite loci for the European eel ( Anguilla anguilla ) |
title_sort |
isolation and characterization of expressed sequence tag‐linked microsatellite loci for the european eel ( anguilla anguilla ) |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2008.02419.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02419.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02419.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Molecular Ecology Resources volume 9, issue 1, page 233-235 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2008.02419.x |
container_title |
Molecular Ecology Resources |
container_volume |
9 |
container_issue |
1 |
container_start_page |
233 |
op_container_end_page |
235 |
_version_ |
1784264703184207872 |