Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S
Abstract We examined ultraviolet radiation (UVR)‐induced DNA damage in marine micro‐organisms collected from surface seawater along a latitudinal transect in the Central Pacific Ocean from 70°N to 68°S. Samples were collected predawn and incubated under ambient UVR in transparent incubators at in si...
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crwiley:10.1111/j.1751-1097.2008.00462.x 2024-03-24T09:00:03+00:00 Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S Meador, Jarah A. Baldwin, Amy J. Catala, Phillipe Jeffrey, Wade H. Joux, Fabien Moss, Joseph A. Dean Pakulski, J. Stevens, Richard Mitchell, David L. 2009 http://dx.doi.org/10.1111/j.1751-1097.2008.00462.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1751-1097.2008.00462.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1751-1097.2008.00462.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Photochemistry and Photobiology volume 85, issue 1, page 412-420 ISSN 0031-8655 1751-1097 Physical and Theoretical Chemistry General Medicine Biochemistry journal-article 2009 crwiley https://doi.org/10.1111/j.1751-1097.2008.00462.x 2024-02-28T02:14:44Z Abstract We examined ultraviolet radiation (UVR)‐induced DNA damage in marine micro‐organisms collected from surface seawater along a latitudinal transect in the Central Pacific Ocean from 70°N to 68°S. Samples were collected predawn and incubated under ambient UVR in transparent incubators at in situ temperatures until late afternoon at which time they were filtered into primarily bacterioplankton and eukaryotic fractions. Cyclobutane pyrimidine dimers (CPDs) and (6‐4) photoproducts [(6‐4)PDs] were quantified in DNA extracts using radioimmunoassays. UVB was lowest in the polar regions and highest near the equator and correlations between UVB and DNA damage were observed. The eukaryotic fraction showed significant CPDs across the entire transect; (6‐4)PDs were detected only in the tropics. The bacterial fraction showed no accumulation of (6‐4)PDs at any latitude, although residual (6‐4)PDs were observed. Bacterial cell volumes were greatest in the sub‐Arctic and northern temperate latitudes and lower in the tropics and southern hemisphere, a unique observation that parallels Bergmann’s rule. A strong negative correlation was observed between cell volume and CPDs. The environmental impact of solar UVR on marine micro‐organisms in the open ocean is complex and our results suggest that several factors such as DNA repair, cell size, temperature, salinity, nutrients and species composition are important in determining relative sensitivity. Article in Journal/Newspaper Arctic Wiley Online Library Arctic Pacific Photochemistry and Photobiology 85 1 412 420 |
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Wiley Online Library |
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English |
topic |
Physical and Theoretical Chemistry General Medicine Biochemistry |
spellingShingle |
Physical and Theoretical Chemistry General Medicine Biochemistry Meador, Jarah A. Baldwin, Amy J. Catala, Phillipe Jeffrey, Wade H. Joux, Fabien Moss, Joseph A. Dean Pakulski, J. Stevens, Richard Mitchell, David L. Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
topic_facet |
Physical and Theoretical Chemistry General Medicine Biochemistry |
description |
Abstract We examined ultraviolet radiation (UVR)‐induced DNA damage in marine micro‐organisms collected from surface seawater along a latitudinal transect in the Central Pacific Ocean from 70°N to 68°S. Samples were collected predawn and incubated under ambient UVR in transparent incubators at in situ temperatures until late afternoon at which time they were filtered into primarily bacterioplankton and eukaryotic fractions. Cyclobutane pyrimidine dimers (CPDs) and (6‐4) photoproducts [(6‐4)PDs] were quantified in DNA extracts using radioimmunoassays. UVB was lowest in the polar regions and highest near the equator and correlations between UVB and DNA damage were observed. The eukaryotic fraction showed significant CPDs across the entire transect; (6‐4)PDs were detected only in the tropics. The bacterial fraction showed no accumulation of (6‐4)PDs at any latitude, although residual (6‐4)PDs were observed. Bacterial cell volumes were greatest in the sub‐Arctic and northern temperate latitudes and lower in the tropics and southern hemisphere, a unique observation that parallels Bergmann’s rule. A strong negative correlation was observed between cell volume and CPDs. The environmental impact of solar UVR on marine micro‐organisms in the open ocean is complex and our results suggest that several factors such as DNA repair, cell size, temperature, salinity, nutrients and species composition are important in determining relative sensitivity. |
format |
Article in Journal/Newspaper |
author |
Meador, Jarah A. Baldwin, Amy J. Catala, Phillipe Jeffrey, Wade H. Joux, Fabien Moss, Joseph A. Dean Pakulski, J. Stevens, Richard Mitchell, David L. |
author_facet |
Meador, Jarah A. Baldwin, Amy J. Catala, Phillipe Jeffrey, Wade H. Joux, Fabien Moss, Joseph A. Dean Pakulski, J. Stevens, Richard Mitchell, David L. |
author_sort |
Meador, Jarah A. |
title |
Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
title_short |
Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
title_full |
Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
title_fullStr |
Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
title_full_unstemmed |
Sunlight‐induced DNA Damage in Marine Micro‐organisms Collected Along a Latitudinal Gradient from 70°N to 68°S |
title_sort |
sunlight‐induced dna damage in marine micro‐organisms collected along a latitudinal gradient from 70°n to 68°s |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1751-1097.2008.00462.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1751-1097.2008.00462.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1751-1097.2008.00462.x |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Photochemistry and Photobiology volume 85, issue 1, page 412-420 ISSN 0031-8655 1751-1097 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1751-1097.2008.00462.x |
container_title |
Photochemistry and Photobiology |
container_volume |
85 |
container_issue |
1 |
container_start_page |
412 |
op_container_end_page |
420 |
_version_ |
1794399927785226240 |