Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella)
ABSTRACT: To make more effective use of underutilized resources, acid‐solubilized collagen (ASC) and pepsin‐solubilized collagen (PSC) were isolated from the skin of deep‐sea redfish ( Sebastes mentella ) and characterized for their potential in commercial applications. The yield of ASC (47.5%) was...
Published in: | Journal of Food Science |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2007
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1750-3841.2007.00478.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3841.2007.00478.x |
id |
crwiley:10.1111/j.1750-3841.2007.00478.x |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1111/j.1750-3841.2007.00478.x 2024-06-23T07:56:44+00:00 Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) Wang, L. An, X. Xin, Z. Zhao, L. Hu, Q. 2007 http://dx.doi.org/10.1111/j.1750-3841.2007.00478.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3841.2007.00478.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 72, issue 8 ISSN 0022-1147 1750-3841 journal-article 2007 crwiley https://doi.org/10.1111/j.1750-3841.2007.00478.x 2024-06-11T04:38:32Z ABSTRACT: To make more effective use of underutilized resources, acid‐solubilized collagen (ASC) and pepsin‐solubilized collagen (PSC) were isolated from the skin of deep‐sea redfish ( Sebastes mentella ) and characterized for their potential in commercial applications. The yield of ASC (47.5%) was lower compared to PSC (92.2%), but the purity of ASC was significantly higher. The intrinsic viscosity of ASC (15.9 dL/g) was greater than PSC (14.6 dL/g), indicating a higher average molecular weight of ASC on account of the high proportion of polymers of collagen. The denaturation temperatures of ASC and PSC were 16.1 and 15.7 °C, respectively, suggesting the triple helical structure of PSC was still predominant. The amino acid profiles of ASC and PSC were similar with lower imino acid content than most other species, which might be the reason for the lower denaturation temperature. SDS‐PAGE and FTIR showed that both ASC and PSC were type I mainly with slight structure differences. ASC held its triple helical structure well, and possessed a higher extent of intermolecular cross‐link. While the structure of PSC was changed slightly due to the loss of N‐ and C‐terminus domains, the triple helical structure was still predominant as a result of the formation of more and/or stronger hydrogen bond. Article in Journal/Newspaper Sebastes mentella Wiley Online Library Journal of Food Science 72 8 E450 E455 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
ABSTRACT: To make more effective use of underutilized resources, acid‐solubilized collagen (ASC) and pepsin‐solubilized collagen (PSC) were isolated from the skin of deep‐sea redfish ( Sebastes mentella ) and characterized for their potential in commercial applications. The yield of ASC (47.5%) was lower compared to PSC (92.2%), but the purity of ASC was significantly higher. The intrinsic viscosity of ASC (15.9 dL/g) was greater than PSC (14.6 dL/g), indicating a higher average molecular weight of ASC on account of the high proportion of polymers of collagen. The denaturation temperatures of ASC and PSC were 16.1 and 15.7 °C, respectively, suggesting the triple helical structure of PSC was still predominant. The amino acid profiles of ASC and PSC were similar with lower imino acid content than most other species, which might be the reason for the lower denaturation temperature. SDS‐PAGE and FTIR showed that both ASC and PSC were type I mainly with slight structure differences. ASC held its triple helical structure well, and possessed a higher extent of intermolecular cross‐link. While the structure of PSC was changed slightly due to the loss of N‐ and C‐terminus domains, the triple helical structure was still predominant as a result of the formation of more and/or stronger hydrogen bond. |
format |
Article in Journal/Newspaper |
author |
Wang, L. An, X. Xin, Z. Zhao, L. Hu, Q. |
spellingShingle |
Wang, L. An, X. Xin, Z. Zhao, L. Hu, Q. Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
author_facet |
Wang, L. An, X. Xin, Z. Zhao, L. Hu, Q. |
author_sort |
Wang, L. |
title |
Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
title_short |
Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
title_full |
Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
title_fullStr |
Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
title_full_unstemmed |
Isolation and Characterization of Collagen from the Skin of Deep‐Sea Redfish ( Sebastes mentella) |
title_sort |
isolation and characterization of collagen from the skin of deep‐sea redfish ( sebastes mentella) |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1750-3841.2007.00478.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3841.2007.00478.x |
genre |
Sebastes mentella |
genre_facet |
Sebastes mentella |
op_source |
Journal of Food Science volume 72, issue 8 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1750-3841.2007.00478.x |
container_title |
Journal of Food Science |
container_volume |
72 |
container_issue |
8 |
container_start_page |
E450 |
op_container_end_page |
E455 |
_version_ |
1802650049431207936 |