Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening
ABSTRACT: Recombinant chum salmon cystatin (RC) expressed in Saccharomyces cerevisiae was purified by His‐select nickel affinity chromatography. The specific inhibitory activities of RC against papain and cathepsin L were 7.45 and 10.24 U/mg, respectively. RC was stable over pH 5.0 to 7.0 and at tem...
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crwiley:10.1111/j.1750-3841.2007.00393.x 2023-12-03T10:08:42+01:00 Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening Li, D.K. Lin, H. Kim, S.M. 2007 http://dx.doi.org/10.1111/j.1750-3841.2007.00393.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3841.2007.00393.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Food Science volume 72, issue 5 ISSN 0022-1147 1750-3841 Food Science journal-article 2007 crwiley https://doi.org/10.1111/j.1750-3841.2007.00393.x 2023-11-09T14:21:18Z ABSTRACT: Recombinant chum salmon cystatin (RC) expressed in Saccharomyces cerevisiae was purified by His‐select nickel affinity chromatography. The specific inhibitory activities of RC against papain and cathepsin L were 7.45 and 10.24 U/mg, respectively. RC was stable over pH 5.0 to 7.0 and at temperature below 65 °C. RC was used to prevent the gel weakening of Alaska pollock surimi. RC at 100 μg/g showed the highest inhibitory activity against the autolysis of surimi based on the analysis of TCA‐soluble peptides. As the concentration of RC increased, both the breaking force and deformation of modori gel greatly increased ( P < 0.05). The addition of RC resulted in less expressible drip, which coincided with the increase of whiteness. More myosin heavy chain (MHC) was retained as the addition of RC increased. Therefore, RC could prevent the degradation of proteins in Alaska pollock surimi and was better than egg white (EW). Thus, RC could be applied to Alaska pollock surimi to prevent gel weakening and RC at 100 μg/g was the optimal concentration. Article in Journal/Newspaper alaska pollock Theragra chalcogramma Alaska Wiley Online Library (via Crossref) Journal of Food Science 72 5 C294 C299 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Food Science |
spellingShingle |
Food Science Li, D.K. Lin, H. Kim, S.M. Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
topic_facet |
Food Science |
description |
ABSTRACT: Recombinant chum salmon cystatin (RC) expressed in Saccharomyces cerevisiae was purified by His‐select nickel affinity chromatography. The specific inhibitory activities of RC against papain and cathepsin L were 7.45 and 10.24 U/mg, respectively. RC was stable over pH 5.0 to 7.0 and at temperature below 65 °C. RC was used to prevent the gel weakening of Alaska pollock surimi. RC at 100 μg/g showed the highest inhibitory activity against the autolysis of surimi based on the analysis of TCA‐soluble peptides. As the concentration of RC increased, both the breaking force and deformation of modori gel greatly increased ( P < 0.05). The addition of RC resulted in less expressible drip, which coincided with the increase of whiteness. More myosin heavy chain (MHC) was retained as the addition of RC increased. Therefore, RC could prevent the degradation of proteins in Alaska pollock surimi and was better than egg white (EW). Thus, RC could be applied to Alaska pollock surimi to prevent gel weakening and RC at 100 μg/g was the optimal concentration. |
format |
Article in Journal/Newspaper |
author |
Li, D.K. Lin, H. Kim, S.M. |
author_facet |
Li, D.K. Lin, H. Kim, S.M. |
author_sort |
Li, D.K. |
title |
Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
title_short |
Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
title_full |
Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
title_fullStr |
Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
title_full_unstemmed |
Application of Recombinant Chum Salmon Cystatin to Alaska Pollock ( Theragra chalcogramma ) Surimi to Prevent Gel Weakening |
title_sort |
application of recombinant chum salmon cystatin to alaska pollock ( theragra chalcogramma ) surimi to prevent gel weakening |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1111/j.1750-3841.2007.00393.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-3841.2007.00393.x |
genre |
alaska pollock Theragra chalcogramma Alaska |
genre_facet |
alaska pollock Theragra chalcogramma Alaska |
op_source |
Journal of Food Science volume 72, issue 5 ISSN 0022-1147 1750-3841 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1750-3841.2007.00393.x |
container_title |
Journal of Food Science |
container_volume |
72 |
container_issue |
5 |
container_start_page |
C294 |
op_container_end_page |
C299 |
_version_ |
1784258988937838592 |