Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA
Please cite this paper as: Verity et al. (2011) Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA. Influenza and Other Respiratory Viruses DOI:10.1111/j.1750‐2659.2011.00273.x. Background Vaccination is considered the most effective means of reducing influenz...
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crwiley:10.1111/j.1750-2659.2011.00273.x 2024-06-23T07:57:25+00:00 Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA Verity, Erin E. Camuglia, Sarina Agius, Catherine T. Ong, Chi Shaw, Robert Barr, Ian Middleton, Deborah Rockman, Steven 2011 http://dx.doi.org/10.1111/j.1750-2659.2011.00273.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-2659.2011.00273.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1750-2659.2011.00273.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Influenza and Other Respiratory Viruses volume 6, issue 2, page 101-109 ISSN 1750-2640 1750-2659 journal-article 2011 crwiley https://doi.org/10.1111/j.1750-2659.2011.00273.x 2024-06-06T04:21:43Z Please cite this paper as: Verity et al. (2011) Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA. Influenza and Other Respiratory Viruses DOI:10.1111/j.1750‐2659.2011.00273.x. Background Vaccination is considered the most effective means of reducing influenza burden. The emergence of H5N1 and pandemic spread of novel H1N1/2009 viruses reinforces the need to have strategies in place to rapidly develop seed viruses for vaccine manufacture. Methods Candidate pandemic vaccine strains consisting of the circulating strain haemagglutinin (HA) and neuraminidase (NA) in an A/PR/8/34 backbone were generated using alternative synthetic DNA approaches, including site‐directed mutagenesis of DNA encoding related virus strains, and rapid generation of virus using synthetic DNA cloned into plasmid vectors. Results Firstly, synthetic A/Bar Headed Goose/Qinghai/1A/2005 (H5N1) virus was generated from an A/Vietnam/1194/2004 template using site‐directed mutagenesis. Secondly, A/Whooper Swan/Mongolia/244/2005 (H5N1) and A/California/04/09 (H1N1) viruses were generated using synthetic DNA encoding the viral HA and NA genes. Replication and antigenicity of the synthetic viruses were comparable to that of the corresponding non‐synthetic viruses. Conclusions In the event of an influenza pandemic, the use of these approaches may significantly reduce the time required to generate and distribute the vaccine seed virus and vaccine manufacture. These approaches also offer the advantage of not needing to handle wild‐type virus, potentially diminishing biocontainment requirements. Article in Journal/Newspaper Whooper Swan Wiley Online Library Influenza and Other Respiratory Viruses 6 2 101 109 |
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Please cite this paper as: Verity et al. (2011) Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA. Influenza and Other Respiratory Viruses DOI:10.1111/j.1750‐2659.2011.00273.x. Background Vaccination is considered the most effective means of reducing influenza burden. The emergence of H5N1 and pandemic spread of novel H1N1/2009 viruses reinforces the need to have strategies in place to rapidly develop seed viruses for vaccine manufacture. Methods Candidate pandemic vaccine strains consisting of the circulating strain haemagglutinin (HA) and neuraminidase (NA) in an A/PR/8/34 backbone were generated using alternative synthetic DNA approaches, including site‐directed mutagenesis of DNA encoding related virus strains, and rapid generation of virus using synthetic DNA cloned into plasmid vectors. Results Firstly, synthetic A/Bar Headed Goose/Qinghai/1A/2005 (H5N1) virus was generated from an A/Vietnam/1194/2004 template using site‐directed mutagenesis. Secondly, A/Whooper Swan/Mongolia/244/2005 (H5N1) and A/California/04/09 (H1N1) viruses were generated using synthetic DNA encoding the viral HA and NA genes. Replication and antigenicity of the synthetic viruses were comparable to that of the corresponding non‐synthetic viruses. Conclusions In the event of an influenza pandemic, the use of these approaches may significantly reduce the time required to generate and distribute the vaccine seed virus and vaccine manufacture. These approaches also offer the advantage of not needing to handle wild‐type virus, potentially diminishing biocontainment requirements. |
format |
Article in Journal/Newspaper |
author |
Verity, Erin E. Camuglia, Sarina Agius, Catherine T. Ong, Chi Shaw, Robert Barr, Ian Middleton, Deborah Rockman, Steven |
spellingShingle |
Verity, Erin E. Camuglia, Sarina Agius, Catherine T. Ong, Chi Shaw, Robert Barr, Ian Middleton, Deborah Rockman, Steven Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
author_facet |
Verity, Erin E. Camuglia, Sarina Agius, Catherine T. Ong, Chi Shaw, Robert Barr, Ian Middleton, Deborah Rockman, Steven |
author_sort |
Verity, Erin E. |
title |
Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
title_short |
Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
title_full |
Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
title_fullStr |
Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
title_full_unstemmed |
Rapid generation of pandemic influenza virus vaccine candidate strains using synthetic DNA |
title_sort |
rapid generation of pandemic influenza virus vaccine candidate strains using synthetic dna |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1111/j.1750-2659.2011.00273.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1750-2659.2011.00273.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1750-2659.2011.00273.x |
genre |
Whooper Swan |
genre_facet |
Whooper Swan |
op_source |
Influenza and Other Respiratory Viruses volume 6, issue 2, page 101-109 ISSN 1750-2640 1750-2659 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1750-2659.2011.00273.x |
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Influenza and Other Respiratory Viruses |
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6 |
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2 |
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101 |
op_container_end_page |
109 |
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1802651045592039424 |