A Novel Cell Line from Liver of Stone Flounder, Kareius bicoloratus , and Its Susceptibility to Different Iridoviruses

• Published in: Journal of the World Aquaculture Society
• Main Authors: Xu, Jianrong, Bao, Zhenmin, Han, Xiaolei
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2011
• Subjects: Agronomy and Crop Science; Aquatic Science; Turbot
• Online Access: http://dx.doi.org/10.1111/j.1749-7345.2010.00445.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1749-7345.2010.00445.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1749-7345.2010.00445.x

A novel cell line (SFL) was established from the liver of stone flounder, Kareius bicoloratus , and its susceptibility to different iridoviruses was evaluated. The SFL cells grew well in Dulbecco's Modified Eagle Medium supplemented with fetal bovine serum, basic fibroblast growth factor, and insulin‐like growth factor‐II, and have been subcultured over 82 passages. The optimal growth temperature was 22 C. The SFL cells were fibroblastic in morphology and grew at a steady rate, with a population doubling time of 38.8 h. Karyotype analysis showed that SFL cells exhibited chromosomal aneuploidy with a modal chromosome number of 48. The susceptibility evaluation of SFL cells revealed that cytopathic effects (CPE) appeared after infection by different iridoviruses, lymphocystis disease virus (LCDV) and turbot reddish body iridovirus (TRBIV). In addition, a large number of TRBIV and LCDV particles were observed in the infected SFL cells by electron microscope examination. It is suggested that the SFL cell line could be used as a valuable tool for isolation and propagation of different iridoviruses.